Detailed information NTG0359535

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EA714_RS14245
• Genome accession: NZ_CP039520
• Coordinates: 2956377..2957495 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain TG22627
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2951377..2962495

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EA714_RS14235 (EA714_014235)	-	2954493..2955185 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
EA714_RS14240 (EA714_014240)	pilT	2955312..2956349 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EA714_RS14245 (EA714_014245)	pilU	2956377..2957495 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EA714_RS14250 (EA714_014250)	fur	2957565..2958002 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EA714_RS14255 (EA714_014255)	-	2958114..2958512 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EA714_RS14260 (EA714_014260)	-	2958567..2958890 (-)	324	WP_000442587.1	RnfH family protein	-
EA714_RS14265 (EA714_014265)	-	2958890..2959957 (-)	1068	WP_002019572.1	hypothetical protein	-
EA714_RS14275 (EA714_014275)	-	2960110..2960565 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EA714_RS14280 (EA714_014280)	-	2960818..2961666 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=359535 EA714_RS14245 WP_000347039.1 2956377..2957495(+) (pilU) [Acinetobacter baumannii strain TG22627]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=359535 EA714_RS14245 WP_000347039.1 2956377..2957495(+) (pilU) [Acinetobacter baumannii strain TG22627]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395