Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   E8M03_RS08240 Genome accession   NZ_CP039448
Coordinates   1661608..1662105 (-) Length   165 a.a.
NCBI ID   WP_001838632.1    Uniprot ID   A0A6A9GZK7
Organism   Staphylococcus aureus strain VGC1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1656608..1667105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M03_RS08215 (E8M03_08305) gcvPB 1656770..1658242 (-) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  E8M03_RS08220 (E8M03_08310) gcvPA 1658235..1659581 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  E8M03_RS08225 (E8M03_08315) gcvT 1659601..1660692 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E8M03_RS08230 (E8M03_08320) - 1660851..1661375 (-) 525 WP_001015116.1 shikimate kinase -
  E8M03_RS08235 (E8M03_08325) - 1661365..1661511 (-) 147 WP_001789879.1 hypothetical protein -
  E8M03_RS08240 (E8M03_08330) comGF 1661608..1662105 (-) 498 WP_001838632.1 competence type IV pilus minor pilin ComGF Machinery gene
  E8M03_RS08245 (E8M03_08335) comGE 1662023..1662322 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E8M03_RS08250 (E8M03_08340) comGD 1662309..1662755 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  E8M03_RS08255 (E8M03_08345) comGC 1662733..1663044 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E8M03_RS08260 (E8M03_08350) comGB 1663058..1664128 (-) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  E8M03_RS08265 (E8M03_08355) comGA 1664100..1665074 (-) 975 WP_000697218.1 competence type IV pilus ATPase ComGA Machinery gene
  E8M03_RS08270 (E8M03_08360) - 1665126..1665749 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E8M03_RS08275 (E8M03_08365) - 1665746..1666075 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E8M03_RS08280 (E8M03_08370) - 1666075..1667061 (-) 987 WP_000161315.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19184.87 Da        Isoelectric Point: 10.2192

>NTDB_id=358668 E8M03_RS08240 WP_001838632.1 1661608..1662105(-) (comGF) [Staphylococcus aureus strain VGC1]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNNIENREQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=358668 E8M03_RS08240 WP_001838632.1 1661608..1662105(-) (comGF) [Staphylococcus aureus strain VGC1]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCTCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACAATATTGAAAATAGGGAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A9GZK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

96.364

100

0.964
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment