Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   E8M03_RS08265 Genome accession   NZ_CP039448
Coordinates   1664100..1665074 (-) Length   324 a.a.
NCBI ID   WP_000697218.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VGC1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1659100..1670074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M03_RS08225 (E8M03_08315) gcvT 1659601..1660692 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  E8M03_RS08230 (E8M03_08320) - 1660851..1661375 (-) 525 WP_001015116.1 shikimate kinase -
  E8M03_RS08235 (E8M03_08325) - 1661365..1661511 (-) 147 WP_001789879.1 hypothetical protein -
  E8M03_RS08240 (E8M03_08330) comGF 1661608..1662105 (-) 498 WP_001838632.1 competence type IV pilus minor pilin ComGF Machinery gene
  E8M03_RS08245 (E8M03_08335) comGE 1662023..1662322 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  E8M03_RS08250 (E8M03_08340) comGD 1662309..1662755 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  E8M03_RS08255 (E8M03_08345) comGC 1662733..1663044 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  E8M03_RS08260 (E8M03_08350) comGB 1663058..1664128 (-) 1071 WP_000776407.1 competence type IV pilus assembly protein ComGB Machinery gene
  E8M03_RS08265 (E8M03_08355) comGA 1664100..1665074 (-) 975 WP_000697218.1 competence type IV pilus ATPase ComGA Machinery gene
  E8M03_RS08270 (E8M03_08360) - 1665126..1665749 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  E8M03_RS08275 (E8M03_08365) - 1665746..1666075 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  E8M03_RS08280 (E8M03_08370) - 1666075..1667061 (-) 987 WP_000161315.1 ROK family glucokinase -
  E8M03_RS08285 (E8M03_08375) - 1667058..1667261 (-) 204 WP_000087561.1 YqgQ family protein -
  E8M03_RS08290 (E8M03_08380) - 1667242..1668705 (-) 1464 WP_001017869.1 rhomboid family intramembrane serine protease -
  E8M03_RS08295 (E8M03_08385) - 1668717..1669256 (-) 540 WP_000164548.1 5-formyltetrahydrofolate cyclo-ligase -
  E8M03_RS08300 (E8M03_08390) rpmG 1669465..1669614 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36970.02 Da        Isoelectric Point: 8.6529

>NTDB_id=358673 E8M03_RS08265 WP_000697218.1 1664100..1665074(-) (comGA) [Staphylococcus aureus strain VGC1]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQELLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=358673 E8M03_RS08265 WP_000697218.1 1664100..1665074(-) (comGA) [Staphylococcus aureus strain VGC1]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGAATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCCTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTAATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAGGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997

Multiple sequence alignment