Detailed information NTG0358168

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EA743_RS20205
• Genome accession: NZ_CP039341
• Coordinates: 3661472..3662332 (+)
• Length: 286 a.a.
• NCBI ID: WP_001152277.1
• Uniprot ID: A0A009HWK1
• Organism: Acinetobacter baumannii strain TG31986
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3656472..3667332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EA743_RS17725 (EA743_017725)	secG	3657077..3657406 (-)	330	WP_000555570.1	preprotein translocase subunit SecG	-
EA743_RS17730 (EA743_017730)	tpiA	3657419..3658213 (-)	795	WP_000016942.1	triose-phosphate isomerase	-
EA743_RS17735 (EA743_017735)	pilB	3658504..3660216 (+)	1713	WP_001274988.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EA743_RS17740 (EA743_017740)	pilC	3660246..3661472 (+)	1227	WP_000279218.1	type II secretion system F family protein	Machinery gene
EA743_RS20205 (EA743_017745)	pilD	3661472..3662332 (+)	861	WP_001152277.1	prepilin peptidase	Machinery gene
EA743_RS17750 (EA743_017750)	coaE	3662334..3662930 (+)	597	WP_000874125.1	dephospho-CoA kinase	-
EA743_RS17755 (EA743_017755)	-	3662927..3663841 (-)	915	WP_024432290.1	DMT family transporter	-
EA743_RS17760 (EA743_017760)	rlmB	3663877..3664626 (-)	750	WP_001118149.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
EA743_RS17765 (EA743_017765)	-	3664728..3665054 (-)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
EA743_RS17770 (EA743_017770)	-	3665131..3666441 (-)	1311	WP_000753281.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32038.43 Da
• Isoelectric Point: 7.1041

>NTDB_id=358168 EA743_RS20205 WP_001152277.1 3661472..3662332(+) (pilD) [Acinetobacter baumannii strain TG31986]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVIVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=358168 EA743_RS20205 WP_001152277.1 3661472..3662332(+) (pilD) [Acinetobacter baumannii strain TG31986]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTGATTTACCGCACGCCAAAAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGCTGCTCA
ATCCTGAGCAACCCATTATTGATCATGAGAAGTTAACTTTAAGTAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCGATTAG
TATTCGCTATCCAGCCATCGAACTACTCACTATGTTATGTTCATTAGTAGTCGTCATAGTGTTTGGTCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCCTACCTTTAGCTGCGCTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGAGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-127)

3D structure

• AlphaFold DB: A0A009HWK1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	98.951	100	0.99
pilD	Acinetobacter nosocomialis M2	98.601	100	0.986
pilD	Vibrio cholerae strain A1552	54.044	95.105	0.514
pilD	Vibrio campbellii strain DS40M4	52.768	94.755	0.5
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441