Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E6A55_RS01955 Genome accession   NZ_CP039287
Coordinates   404279..405760 (-) Length   493 a.a.
NCBI ID   WP_011614486.1    Uniprot ID   P28615
Organism   Cupriavidus necator H16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399279..410760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6A55_RS01925 (E6A55_01925) - 399597..400256 (-) 660 WP_010814381.1 LON peptidase substrate-binding domain-containing protein -
  E6A55_RS01930 (E6A55_01930) rapZ 400348..401232 (-) 885 WP_010814382.1 RNase adapter RapZ -
  E6A55_RS01935 (E6A55_01935) - 401351..401752 (-) 402 WP_011614484.1 PsiF family protein -
  E6A55_RS01940 (E6A55_01940) hprK 401915..402886 (-) 972 WP_010814384.1 HPr(Ser) kinase/phosphatase -
  E6A55_RS01945 (E6A55_01945) ptsN 402968..403423 (-) 456 WP_010814385.1 PTS IIA-like nitrogen regulatory protein PtsN -
  E6A55_RS01950 (E6A55_01950) hpf 403880..404230 (-) 351 WP_010814386.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  E6A55_RS01955 (E6A55_01955) rpoN/rpoN1 404279..405760 (-) 1482 WP_011614486.1 RNA polymerase factor sigma-54 Machinery gene
  E6A55_RS01960 (E6A55_01960) lptB 405895..406695 (-) 801 WP_010814388.1 LPS export ABC transporter ATP-binding protein -
  E6A55_RS01965 (E6A55_01965) lptA 406764..407414 (-) 651 WP_011614488.1 lipopolysaccharide transport periplasmic protein LptA -
  E6A55_RS01970 (E6A55_01970) lptC 407482..408111 (-) 630 WP_010814390.1 LPS export ABC transporter periplasmic protein LptC -
  E6A55_RS01975 (E6A55_01975) - 408155..409138 (-) 984 WP_010814391.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54770.07 Da        Isoelectric Point: 4.9195

>NTDB_id=357874 E6A55_RS01955 WP_011614486.1 404279..405760(-) (rpoN/rpoN1) [Cupriavidus necator H16]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEARADGAADDDSYGDSGNGDDYGSSDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISARDKGLAI
FLIESLDDDGYLSASLEEICTELPEELEFEIEEVHAILTLLQSFDPPGVGARNAAECLALQLRRLTHPQRELALNIVTNH
LELLAVRDYTRLKKALQVDEAALKSAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKGGGGWIAQLNPDVMPRLRINDMY
AQILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDKILRVSQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=357874 E6A55_RS01955 WP_011614486.1 404279..405760(-) (rpoN/rpoN1) [Cupriavidus necator H16]
ATGAAACCGTCGCTACAGCTCCGCCTTTCCCAGCATCTGGCCCTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTGGAACTGCAGCAAGAGGTCGAACAGGCACTGACGGAAAACCCTCTGCTCGAACGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCGGCCGACGGCTCGGTCAACCTGCAGAGCGCACCGGCGCCCGCGCCGGCAGAGCCGCAG
GGCAATGGCGAGGCCCGCGCCGACGGCGCGGCTGACGACGACAGCTATGGCGACAGCGGCAACGGCGACGACTATGGCAG
CAGCGACTGGAGCCTGGACGACTTTGCCCGCCGCCCCCAGGGCGACGAGGACGAAAAAACGCCGATGCAGCTGCGCGAAG
CCGAGCCCACGCTGCGCGAGTACCTGATGGAACAGCTCACGCCGCTGAAGATCTCGGCGCGCGACAAGGGCCTGGCCATC
TTCCTGATCGAATCGCTCGACGACGATGGCTACCTGAGCGCATCGCTTGAGGAGATCTGCACGGAGTTGCCGGAAGAACT
CGAGTTCGAGATCGAGGAGGTCCACGCCATCCTCACGCTGCTGCAGAGCTTCGACCCGCCCGGCGTGGGCGCGCGCAACG
CCGCCGAGTGCCTGGCCCTGCAGTTGCGGCGCCTGACGCACCCGCAGCGCGAACTGGCCCTGAACATCGTGACCAACCAC
CTGGAGTTGCTCGCAGTACGCGACTACACGCGGCTGAAGAAGGCGCTGCAGGTGGATGAAGCGGCGCTGAAGTCCGCACA
CGAACTGATCCGCTCGCTGGCGCCCTACCCCGGCCATGCATACAGCCGCCCGGAGGCGGACTTCGTGGTGCCGGACGTGT
TCGTGCGCAAGGGTGGCGGCGGCTGGATCGCGCAGCTCAATCCGGATGTGATGCCGAGGCTGCGCATCAATGACATGTAT
GCGCAAATCCTACGTGGCGCAAAGGGTGAGTCCGGCACCGCCGGGCTGCAGCAGAAGCTGCAAGAGGCGCGCTGGCTGAT
CAAGAACATCCAGCAGAGGTTCGACAAAATCCTGCGTGTCTCGCAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCAGCC
ACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCAACCATCTCCCGG
GTGACGACCAATAAATATATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGCAGCCACGTGTCCACCGA
AACCGGTGGCGCGGCTTCGTCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCC
TTTCCGACAGTCGCATTGCCGAACTGCTGGGCGAACAAGGCTTCGTTGTCGCACGCCGCACCGTTGCCAAGTATCGCGAA
GCCCTGAAGATCCCCGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P28615

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.242

100

0.805

Multiple sequence alignment