Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   E5790_RS14080 Genome accession   NZ_CP039255
Coordinates   3017962..3019020 (+) Length   352 a.a.
NCBI ID   WP_014038321.1    Uniprot ID   A0AAP7J1E4
Organism   Stenotrophomonas sp. PAMC25021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3012962..3024020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5790_RS14065 (E5790_14065) - 3013379..3014125 (+) 747 WP_111206039.1 hypothetical protein -
  E5790_RS14070 (E5790_14070) - 3014465..3015109 (-) 645 WP_111206040.1 hypothetical protein -
  E5790_RS14075 (E5790_14075) - 3015322..3017742 (-) 2421 WP_046432301.1 penicillin-binding protein 1A -
  E5790_RS14080 (E5790_14080) pilM 3017962..3019020 (+) 1059 WP_014038321.1 pilus assembly protein PilM Machinery gene
  E5790_RS14085 (E5790_14085) - 3019020..3019901 (+) 882 WP_014648259.1 PilN domain-containing protein -
  E5790_RS14090 (E5790_14090) pilO 3019898..3020554 (+) 657 WP_014648258.1 type 4a pilus biogenesis protein PilO -
  E5790_RS14095 (E5790_14095) - 3020554..3021087 (+) 534 WP_005418914.1 pilus assembly protein PilP -
  E5790_RS14100 (E5790_14100) - 3021109..3023079 (+) 1971 WP_032962161.1 type IV pilus secretin PilQ family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37741.35 Da        Isoelectric Point: 4.4006

>NTDB_id=357673 E5790_RS14080 WP_014038321.1 3017962..3019020(+) (pilM) [Stenotrophomonas sp. PAMC25021]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPADLDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGSIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=357673 E5790_RS14080 WP_014038321.1 3017962..3019020(+) (pilM) [Stenotrophomonas sp. PAMC25021]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTCGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGCGGCAATCGTTTCCGCGTGGAACATTACGCTGTGGAACCTCTGCCGCCCAATGCGGTGGTGGAGAAGAACATCG
TTGAAGTGGAGGCCGTGGGTGAGGCCATCCGTCGGGCGATGAACCGTTCAGGCAGCAAGGCCAAATTGGCCGCTGCCGCC
GTCGCGGGGTCGGCAGTGATCACCAAGGTGATCCCGATGCCGGCCGATCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGTCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAATGTCGAGCTGCGCCAGTCCGCGCTGGAACTCGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTACGCTCTGGTCGCCAGCGAGCTGCCGGT
ATCCATCGACGGCGTGGTCGCGCTGGTCGACATCGGCGCCACCATGACCACGCTCAATGTCCTGCGCGGCGGCCGCAGCC
TCTACAGCCGCGAGCAGGTGTTCGGTGGCAAGCAGCTGACCGATGAGATCATGCGCCGCTACGGGCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGCCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCAGGCAGCGAATTCAACCGCGTCGATCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTCGGTGGCCTGCCGGAGATGGTCGAGGAACAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCGCATGCGTTGGCCCAGGATGCCCCCGCGCTGATGATCGCGACCGGTCTGGC
GCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.863

99.716

0.477

Multiple sequence alignment