Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E5U06_RS04720 Genome accession   NZ_CP038871
Coordinates   991365..991799 (+) Length   144 a.a.
NCBI ID   WP_061406215.1    Uniprot ID   Q9LA12
Organism   Pasteurella multocida strain FCf15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 986365..996799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5U06_RS04690 (E5U06_04690) tadA 986475..986975 (-) 501 WP_005722410.1 tRNA adenosine(34) deaminase TadA -
  E5U06_RS04695 (E5U06_04695) - 986990..987841 (-) 852 WP_005722413.1 thymidylate synthase -
  E5U06_RS04700 (E5U06_04700) lgt 987838..988650 (-) 813 WP_135961479.1 prolipoprotein diacylglyceryl transferase -
  E5U06_RS04705 (E5U06_04705) - 988660..989454 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  E5U06_RS04710 (E5U06_04710) rppH 989457..990053 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  E5U06_RS04715 (E5U06_04715) ampD 990639..991205 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E5U06_RS04720 (E5U06_04720) pilA 991365..991799 (+) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  E5U06_RS04725 (E5U06_04725) - 991799..993180 (+) 1382 Protein_920 GspE/PulE family protein -
  E5U06_RS04730 (E5U06_04730) - 993180..994391 (+) 1212 WP_005722560.1 type II secretion system F family protein -
  E5U06_RS04735 (E5U06_04735) - 994391..995113 (+) 723 WP_005722562.1 A24 family peptidase -
  E5U06_RS04740 (E5U06_04740) coaE 995128..995748 (+) 621 WP_005722564.1 dephospho-CoA kinase -
  E5U06_RS04745 (E5U06_04745) yacG 995759..995962 (+) 204 WP_005722566.1 DNA gyrase inhibitor YacG -
  E5U06_RS04750 (E5U06_04750) - 995962..996231 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15213.68 Da        Isoelectric Point: 9.1215

>NTDB_id=356096 E5U06_RS04720 WP_061406215.1 991365..991799(+) (pilA) [Pasteurella multocida strain FCf15]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNTPSECSAGQHGIR
ANISSGKKYLSSINVKSGVITVIGQKSLEGVNYTLSATGNAVDGVSWSVKCNGGSEVFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=356096 E5U06_RS04720 WP_061406215.1 991365..991799(+) (pilA) [Pasteurella multocida strain FCf15]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATACGCCCAGTGAATGTAGTGCAGGACAGCATGGTATTAGA
GCAAATATAAGTTCTGGTAAAAAATATCTCAGCTCAATCAATGTCAAGTCTGGCGTGATTACTGTCATTGGTCAAAAAAG
CTTAGAGGGAGTCAATTACACTTTAAGCGCGACAGGAAATGCCGTTGATGGTGTTTCATGGAGTGTAAAATGTAATGGTG
GTAGTGAAGTTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9LA12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

59.028

100

0.59
  pilA Haemophilus influenzae Rd KW20

59.028

100

0.59
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  comP Acinetobacter baylyi ADP1

37.681

95.833

0.361

Multiple sequence alignment