Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   E5U06_RS00300 Genome accession   NZ_CP038871
Coordinates   53552..54886 (-) Length   444 a.a.
NCBI ID   WP_061406404.1    Uniprot ID   -
Organism   Pasteurella multocida strain FCf15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 48552..59886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5U06_RS00275 (E5U06_00275) secM 50071..50385 (-) 315 WP_010907080.1 secA translation cis-regulator SecM -
  E5U06_RS00280 (E5U06_00280) - 50475..50780 (+) 306 WP_005723691.1 DUF721 domain-containing protein -
  E5U06_RS00285 (E5U06_00285) - 50828..51733 (-) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  E5U06_RS00290 (E5U06_00290) aroB 51737..52825 (-) 1089 WP_061406405.1 3-dehydroquinate synthase -
  E5U06_RS00295 (E5U06_00295) aroK 52842..53369 (-) 528 WP_005717623.1 shikimate kinase AroK -
  E5U06_RS00300 (E5U06_00300) comE 53552..54886 (-) 1335 WP_061406404.1 type IV pilus secretin PilQ Machinery gene
  E5U06_RS00305 (E5U06_00305) - 54896..55258 (-) 363 WP_014668625.1 pilus assembly protein PilP -
  E5U06_RS00310 (E5U06_00310) - 55261..55785 (-) 525 WP_061406403.1 hypothetical protein -
  E5U06_RS00315 (E5U06_00315) - 55785..56300 (-) 516 WP_064972571.1 competence protein ComB -
  E5U06_RS00320 (E5U06_00320) - 56340..57113 (-) 774 WP_061406402.1 pilus assembly protein PilM -
  E5U06_RS00325 (E5U06_00325) - 57249..59810 (+) 2562 WP_061406401.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49240.64 Da        Isoelectric Point: 8.0315

>NTDB_id=356082 E5U06_RS00300 WP_061406404.1 53552..54886(-) (comE) [Pasteurella multocida strain FCf15]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=356082 E5U06_RS00300 WP_061406404.1 53552..54886(-) (comE) [Pasteurella multocida strain FCf15]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTTGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCTGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACAG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCATTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAAAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(264-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.037

97.297

0.604
  comE Haemophilus influenzae 86-028NP

61.343

97.297

0.597
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.806

92.793

0.369
  pilQ Vibrio cholerae strain A1552

39.806

92.793

0.369

Multiple sequence alignment