Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GPY55_RS11020 Genome accession   NZ_CP046831
Coordinates   380948..381412 (-) Length   154 a.a.
NCBI ID   WP_023585961.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 2012AW-0224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375948..386412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY55_RS11000 coaE 376466..377080 (-) 615 WP_031847490.1 dephospho-CoA kinase -
  GPY55_RS11005 pilD 377081..377950 (-) 870 WP_025533026.1 A24 family peptidase Machinery gene
  GPY55_RS11010 pilC 378015..379238 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  GPY55_RS11015 pilB 379263..380948 (-) 1686 WP_031847489.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY55_RS11020 pilA 380948..381412 (-) 465 WP_023585961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY55_RS11025 nadC 381676..382563 (-) 888 WP_031847488.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY55_RS11030 ampD 382656..383207 (+) 552 WP_020841256.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY55_RS11035 pdhR 383613..384380 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15734.80 Da        Isoelectric Point: 4.1438

>NTDB_id=356052 GPY55_RS11020 WP_023585961.1 380948..381412(-) (pilA) [Vibrio parahaemolyticus strain 2012AW-0224]
MKHSKQKKQQGFTLIELMIVVAIIGVLSAFAIPAYSDYTQRTRVAGAAAGISGFKTAVAMCAQDLGQLTGCDQATNDIPA
TIAAGNDGATISYVDAVTVADGVITMVTTGVDDTGTLLELEFTPTIGNGVVQWDIAGTGCSDTTPGRGIDCSGN

Nucleotide


Download         Length: 465 bp        

>NTDB_id=356052 GPY55_RS11020 WP_023585961.1 380948..381412(-) (pilA) [Vibrio parahaemolyticus strain 2012AW-0224]
ATGAAACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTCGTGGCGATTATCGGGGTATT
ATCTGCTTTTGCTATCCCAGCATACTCAGACTACACACAACGTACGCGAGTAGCCGGAGCCGCGGCTGGTATTAGCGGTT
TCAAAACAGCAGTTGCAATGTGCGCACAGGATCTTGGCCAGCTTACAGGCTGCGACCAAGCTACTAATGATATTCCAGCA
ACAATCGCTGCTGGTAATGACGGCGCGACTATTTCATATGTAGATGCGGTTACCGTCGCTGACGGCGTTATTACAATGGT
AACTACAGGTGTTGATGATACAGGTACTTTACTAGAACTTGAGTTTACTCCAACTATTGGCAACGGTGTCGTTCAGTGGG
ATATTGCCGGTACAGGATGTAGCGACACTACTCCTGGCCGCGGCATTGATTGTTCTGGTAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

47.899

77.273

0.37
  pilA2 Legionella pneumophila str. Paris

42.537

87.013

0.37
  pilA2 Legionella pneumophila strain ERS1305867

42.537

87.013

0.37

Multiple sequence alignment