Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   E5Q53_RS08315 Genome accession   NZ_CP038817
Coordinates   1714684..1715979 (+) Length   431 a.a.
NCBI ID   WP_005707305.1    Uniprot ID   A0AAE6JSK3
Organism   Haemophilus parahaemolyticus strain NEB129     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1709684..1720979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q53_RS08290 (E5Q53_08285) - 1709808..1712405 (-) 2598 WP_005707269.1 penicillin-binding protein 1A -
  E5Q53_RS08295 (E5Q53_08290) - 1712545..1713219 (+) 675 WP_005707291.1 hypothetical protein -
  E5Q53_RS08300 (E5Q53_08295) - 1713210..1713731 (+) 522 WP_039863405.1 hypothetical protein -
  E5Q53_RS08305 (E5Q53_08300) - 1713733..1714254 (+) 522 WP_005707388.1 hypothetical protein -
  E5Q53_RS08310 (E5Q53_08305) - 1714256..1714666 (+) 411 WP_005707271.1 hypothetical protein -
  E5Q53_RS08315 (E5Q53_08310) comE 1714684..1715979 (+) 1296 WP_005707305.1 type IV pilus secretin PilQ Machinery gene
  E5Q53_RS08320 (E5Q53_08315) nusB 1716047..1716481 (+) 435 WP_005707302.1 transcription antitermination factor NusB -
  E5Q53_RS08325 (E5Q53_08320) thiL 1716554..1717515 (+) 962 Protein_1588 thiamine-phosphate kinase -
  E5Q53_RS08330 (E5Q53_08325) - 1717531..1718007 (+) 477 WP_005707330.1 phosphatidylglycerophosphatase A -
  E5Q53_RS08335 (E5Q53_08330) - 1718009..1718641 (+) 633 WP_005707390.1 LysE family transporter -
  E5Q53_RS08340 (E5Q53_08335) - 1718671..1719672 (-) 1002 WP_005707375.1 TerC/Alx family metal homeostasis membrane protein -
  E5Q53_RS08345 (E5Q53_08340) - 1719688..1720320 (-) 633 WP_005707367.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 431 a.a.        Molecular weight: 47980.98 Da        Isoelectric Point: 8.8860

>NTDB_id=355778 E5Q53_RS08315 WP_005707305.1 1714684..1715979(+) (comE) [Haemophilus parahaemolyticus strain NEB129]
MKKWLLLFSVFPLSLLANQVSLVLKDAPTAEVVSYLASEMGKNIVLHQDIESKSNIRIENSQFDAILKSIAKINKLNIQQ
ENGIYYISEKTKEDLNEKIKLINEEQKPQLVTRTVKLKYAKASEVIDSLTKGSGSFLNDGGYIHFDERSNSLIIKENAAS
IKNTLALIQELDKPTEQIAIEARIVTISSKNLQELGVRWGMFSADSRIEGVARNSGRIQDLNVNFPVVTGASATLQVASI
NSKLLDLELSALEQENSVEIIASPRLLTTNKKLASIKQGTEIPYTIYDRKSETYDISFKEAVLGLEVTPHISENNQILMD
LVVTQNSQGQSVGNSNSGNTVIAIDKQELNTQVFAKHGETIVLGGVFQHLKSKDSDKIPVLGSIPFIKRLFSYQKDQINK
RELVIFVTPYIVQSKDVAVDFSKTKEKSPKR

Nucleotide


Download         Length: 1296 bp        

>NTDB_id=355778 E5Q53_RS08315 WP_005707305.1 1714684..1715979(+) (comE) [Haemophilus parahaemolyticus strain NEB129]
ATGAAAAAGTGGCTATTATTATTTTCAGTTTTTCCACTCTCGCTATTAGCAAACCAAGTTTCGCTCGTGTTAAAGGATGC
TCCGACAGCCGAAGTTGTTTCTTATTTAGCCAGTGAAATGGGTAAAAATATTGTATTGCATCAAGATATTGAAAGTAAAA
GTAATATTCGTATTGAAAATAGCCAGTTTGATGCAATTTTAAAAAGCATTGCCAAAATCAATAAACTCAATATTCAACAA
GAAAATGGCATCTATTATATTAGTGAAAAAACAAAAGAGGATTTAAACGAAAAAATAAAGCTTATTAATGAAGAACAAAA
GCCACAATTAGTCACTCGTACGGTGAAATTAAAATATGCAAAAGCCTCCGAAGTCATTGATTCACTCACTAAGGGAAGTG
GATCATTTTTAAATGATGGTGGCTATATTCATTTTGATGAGCGAAGCAATAGTTTAATTATTAAGGAAAATGCTGCATCC
ATTAAAAATACGTTAGCTTTAATTCAAGAGCTAGATAAACCAACAGAACAAATTGCCATTGAAGCCCGTATTGTGACCAT
CAGCAGTAAAAACTTGCAAGAACTTGGTGTACGTTGGGGGATGTTCTCCGCAGATAGTCGTATTGAAGGAGTAGCAAGAA
ATAGTGGGCGAATACAAGATTTAAACGTGAATTTTCCCGTGGTTACAGGTGCTTCAGCGACGTTACAAGTCGCTTCCATC
AATTCTAAATTATTGGATTTAGAACTCTCTGCTCTTGAGCAAGAAAACAGTGTAGAAATTATCGCAAGCCCCCGTTTGCT
GACGACTAACAAAAAATTGGCAAGCATTAAACAAGGAACGGAAATCCCTTATACAATTTATGATCGCAAATCGGAAACCT
ACGATATTTCTTTTAAAGAAGCGGTTTTAGGCTTAGAGGTGACGCCGCATATTTCAGAAAATAATCAAATTTTGATGGAT
TTAGTCGTTACCCAAAATTCGCAAGGGCAATCGGTTGGTAATTCTAACTCAGGCAACACCGTAATTGCGATTGATAAACA
AGAACTGAATACGCAAGTATTCGCTAAACACGGTGAAACTATAGTTCTTGGTGGAGTGTTCCAACATTTGAAATCAAAAG
ATAGCGATAAAATTCCTGTGCTTGGTTCAATTCCGTTTATCAAGCGGTTATTTAGCTATCAAAAAGATCAAATTAATAAA
CGTGAATTGGTGATTTTTGTGACGCCTTATATTGTGCAATCAAAAGATGTTGCGGTGGATTTCTCGAAAACGAAAGAAAA
AAGCCCAAAACGATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(251-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

59.059

98.608

0.582
  comE Haemophilus influenzae Rd KW20

49.886

100

0.506
  comE Haemophilus influenzae 86-028NP

49.428

100

0.501
  pilQ Vibrio campbellii strain DS40M4

38.318

99.304

0.381
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.795

96.288

0.374
  pilQ Vibrio cholerae strain A1552

38.795

96.288

0.374

Multiple sequence alignment