Detailed information NTG0355742

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KAN01_RS14150
• Genome accession: NZ_CP038816
• Coordinates: 2903595..2904713 (+)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain KAN01
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2898595..2909713

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KAN01_RS14135 (KAN01_14135)	-	2900761..2901603 (-)	843	WP_006581385.1	undecaprenyl-diphosphate phosphatase	-
KAN01_RS14140 (KAN01_14140)	-	2901711..2902403 (-)	693	WP_032010894.1	YggS family pyridoxal phosphate-dependent enzyme	-
KAN01_RS14145 (KAN01_14145)	pilT	2902530..2903567 (+)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
KAN01_RS14150 (KAN01_14150)	pilU	2903595..2904713 (+)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KAN01_RS14155 (KAN01_14155)	fur	2904783..2905220 (-)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
KAN01_RS14160 (KAN01_14160)	-	2905332..2905730 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KAN01_RS14165 (KAN01_14165)	-	2905786..2906109 (-)	324	WP_032010899.1	RnfH family protein	-
KAN01_RS14170 (KAN01_14170)	-	2906109..2907176 (-)	1068	WP_004710227.1	hypothetical protein	-
KAN01_RS14180 (KAN01_14180)	-	2907329..2907784 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KAN01_RS14185 (KAN01_14185)	-	2908037..2908885 (-)	849	WP_009505210.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=355742 KAN01_RS14150 WP_002052019.1 2903595..2904713(+) (pilU) [Acinetobacter nosocomialis strain KAN01]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=355742 KAN01_RS14150 WP_002052019.1 2903595..2904713(+) (pilU) [Acinetobacter nosocomialis strain KAN01]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCCGATCTGTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTGATTGGACAGCTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGTATTGATACAGACTCATTTGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCAGATGTCATCTTAATTGGTGAAATCCGTTCTCGCGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
GCAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTCGACTTATATAAAGCAGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAGCTTGCAAATGAAGGGCCTGATCAAATAACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403