Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GPX93_RS12355 Genome accession   NZ_CP046749
Coordinates   2709457..2710968 (-) Length   503 a.a.
NCBI ID   WP_000138174.1    Uniprot ID   P37093
Organism   Vibrio cholerae strain 2015V-1118     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2704457..2715968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPX93_RS12325 gspK 2705140..2706150 (-) 1011 WP_053034354.1 type II secretion system minor pseudopilin GspK -
  GPX93_RS12330 gspJ 2706140..2706808 (-) 669 WP_123163039.1 type II secretion system minor pseudopilin GspJ -
  GPX93_RS12335 gspI 2706795..2707148 (-) 354 WP_000837699.1 type II secretion system minor pseudopilin GspI -
  GPX93_RS12340 gspH 2707138..2707722 (-) 585 WP_123163040.1 type II secretion system minor pseudopilin GspH -
  GPX93_RS12345 gspG 2707756..2708196 (-) 441 WP_123163041.1 type II secretion system major pseudopilin GspG -
  GPX93_RS12350 gspF 2708237..2709457 (-) 1221 WP_123163042.1 type II secretion system inner membrane protein GspF -
  GPX93_RS12355 pilB/pilB1 2709457..2710968 (-) 1512 WP_000138174.1 type II secretion system ATPase GspE Machinery gene
  GPX93_RS12360 gspD 2710965..2712992 (-) 2028 WP_019829126.1 type II secretion system secretin GspD -
  GPX93_RS12365 gspC 2713038..2713955 (-) 918 WP_123163043.1 type II secretion system protein GspC -
  GPX93_RS12370 hslR 2714185..2714562 (+) 378 WP_000098851.1 ribosome-associated heat shock protein Hsp15 -
  GPX93_RS12375 hslO 2714596..2715471 (+) 876 WP_001911276.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 56358.58 Da        Isoelectric Point: 6.2107

>NTDB_id=354886 GPX93_RS12355 WP_000138174.1 2709457..2710968(-) (pilB/pilB1) [Vibrio cholerae strain 2015V-1118]
MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVY
QRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGV
LREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLG
MTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLR
AILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDC
KEPYEADKEQRKLFDSKKKEPLILYRATGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRD
DGLDKVRQGITSLEEVMRVTKES

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=354886 GPX93_RS12355 WP_000138174.1 2709457..2710968(-) (pilB/pilB1) [Vibrio cholerae strain 2015V-1118]
ATGACCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTT
GGTGCTGGATTGGAATGAGGATTTCTCCCAAGCCAGCATTTATTACTTAGCGCCGTTGTCGATGGAGGCGCTCGTCGAAA
CCAAGCGGGTGGTCAAGCACGCTTTCCAACTGATTGAGCTCTCTCAAGCGGAGTTTGAAAGCAAGCTAACCCAAGTCTAT
CAGCGTGATTCTTCAGAAGCGCGCCAATTGATGGAAGACATTGGTGCCGACAGTGATGACTTCTTCTCACTGGCGGAAGA
GCTGCCTCAAAACGAAGATTTGCTAGAAAGTGAAGATGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAG
CGATCAAAGAAGGCGCGTCAGATATTCATATTGAAACTTTTGAAAAAACACTGTCGATCCGTTTTCGAGTCGATGGCGTG
CTGCGCGAAGTACTGGCACCCAGCCGCAAACTCTCATCGCTGCTCGTTTCACGGGTCAAAGTGATGGCCAAGCTCGACAT
TGCCGAAAAACGTGTGCCGCAAGATGGCCGTATTTCGCTGCGTATCGGTGGCCGCGCGGTCGATGTGCGGGTTTCAACCA
TGCCTTCATCACATGGTGAGCGAGTGGTAATGCGTTTGCTGGATAAAAACGCGACGCGCCTTGATCTGCACAGTTTGGGT
ATGACGGCGCATAACCATGATAACTTCCGCCGTTTGATTAAGCGGCCGCATGGGATCATTCTGGTGACTGGTCCAACCGG
CTCGGGTAAATCGACCACCTTGTACGCGGGTTTGCAAGAGCTCAACAGCAGCGAGCGCAACATTTTAACCGTAGAAGATC
CGATCGAATTTGATATTGATGGTATCGGTCAGACCCAAGTCAACCCAAGGGTAGACATGACCTTCGCGCGGGGGCTACGC
GCTATTTTGCGTCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGTGACTTGGAAACGGCGCAAATTGCAGTGCAAGC
CTCGCTGACCGGTCACTTAGTGATGTCGACTCTGCACACCAACACGGCCGTTGGGGCGGTGACTCGGCTGCGCGATATGG
GCATCGAGCCTTTTTTGATCTCTTCTTCATTGCTCGGTGTTCTGGCTCAGCGTTTGGTGCGCACCTTATGCCCAGATTGT
AAAGAGCCTTACGAGGCGGACAAAGAGCAGCGCAAACTGTTTGATAGCAAGAAAAAAGAGCCACTAATTCTTTATCGTGC
AACGGGCTGCCCTAAATGTAACCACAAAGGTTACCGTGGCCGAACCGGTATCCACGAGCTGCTGCTGGTGGATGACGCGT
TGCAGGAGCTGATCCATAGCGAGGCGGGCGAACAGGCGATGGAGAAACACATTCGCGCGACCACGCCGAGCATTCGTGAT
GATGGTCTAGATAAGGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATGCGGGTGACTAAGGAGTCCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1P9R
  PDB 1P9W
  PDB 2BH1
  PDB 4KSR
  PDB 4KSS

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.412

100

0.431
  pilB Legionella pneumophila strain ERS1305867

47.126

86.481

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

44.619

88.668

0.396
  pilB Vibrio campbellii strain DS40M4

44.118

87.873

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.745

77.932

0.388
  pilB Vibrio cholerae strain A1552

48.485

78.728

0.382
  pilF Thermus thermophilus HB27

47.761

79.92

0.382
  pilB Acinetobacter baumannii D1279779

46.21

81.312

0.376

Multiple sequence alignment