Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GPW86_RS11900 Genome accession   NZ_CP046742
Coordinates   1321060..1322571 (-) Length   503 a.a.
NCBI ID   WP_000138174.1    Uniprot ID   P37093
Organism   Vibrio cholerae strain 3523-03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1316060..1327571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW86_RS11870 gspK 1316746..1317756 (-) 1011 WP_001201881.1 type II secretion system minor pseudopilin GspK -
  GPW86_RS11875 gspJ 1317746..1318411 (-) 666 WP_000269337.1 type II secretion system minor pseudopilin GspJ -
  GPW86_RS11880 gspI 1318398..1318751 (-) 354 WP_123011192.1 type II secretion system minor pseudopilin GspI -
  GPW86_RS11885 gspH 1318741..1319325 (-) 585 WP_057555103.1 type II secretion system minor pseudopilin GspH -
  GPW86_RS11890 gspG 1319359..1319799 (-) 441 WP_000738789.1 type II secretion system major pseudopilin GspG -
  GPW86_RS11895 gspF 1319840..1321060 (-) 1221 WP_000718695.1 type II secretion system inner membrane protein GspF -
  GPW86_RS11900 pilB/pilB1 1321060..1322571 (-) 1512 WP_000138174.1 type II secretion system ATPase GspE Machinery gene
  GPW86_RS11905 gspD 1322568..1324595 (-) 2028 WP_001958986.1 type II secretion system secretin GspD -
  GPW86_RS11910 gspC 1324641..1325558 (-) 918 WP_123011193.1 type II secretion system protein GspC -
  GPW86_RS11915 hslR 1325788..1326165 (+) 378 WP_000098851.1 ribosome-associated heat shock protein Hsp15 -
  GPW86_RS11920 hslO 1326199..1327074 (+) 876 WP_032481712.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 56358.58 Da        Isoelectric Point: 6.2107

>NTDB_id=354589 GPW86_RS11900 WP_000138174.1 1321060..1322571(-) (pilB/pilB1) [Vibrio cholerae strain 3523-03]
MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVY
QRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGV
LREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLG
MTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLR
AILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDC
KEPYEADKEQRKLFDSKKKEPLILYRATGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRD
DGLDKVRQGITSLEEVMRVTKES

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=354589 GPW86_RS11900 WP_000138174.1 1321060..1322571(-) (pilB/pilB1) [Vibrio cholerae strain 3523-03]
ATGACCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTT
GGTGCTGGATTGGAATGAGGATTTCTCCCAAGCCAGCATTTATTACTTAGCGCCGTTGTCGATGGAGGCGCTCGTCGAAA
CCAAGCGGGTGGTCAAGCACGCTTTCCAACTGATTGAGCTCTCTCAAGCGGAGTTTGAAAGCAAGCTAACCCAAGTCTAT
CAGCGTGATTCTTCAGAAGCGCGTCAATTGATGGAAGACATTGGTGCCGACAGTGATGACTTCTTCTCACTGGCGGAAGA
GCTGCCTCAAAACGAAGATTTGCTAGAAAGTGAAGATGATGCGCCGATCATCAAACTGATCAACGCCATGTTGGGCGAAG
CGATCAAAGAAGGCGCGTCAGATATTCATATTGAAACTTTTGAAAAAACGCTGTCGATCCGTTTTCGAGTCGATGGCGTG
CTGCGCGAAGTACTGGCACCCAGCCGCAAACTCTCATCGCTGCTCGTTTCACGGGTCAAGGTGATGGCCAAGCTCGACAT
TGCCGAAAAACGTGTGCCGCAAGATGGCCGTATTTCGCTGCGTATTGGTGGCCGCGCGGTCGATGTGCGGGTTTCAACCA
TGCCTTCATCACATGGTGAGCGAGTGGTAATGCGTTTGCTGGATAAAAACGCGACGCGCCTTGATCTGCACAGTTTGGGT
ATGACGGCGCATAACCATGATAACTTCCGCCGTTTGATTAAGCGGCCGCATGGGATCATTCTGGTGACTGGCCCAACCGG
CTCGGGTAAATCGACCACCTTGTACGCGGGTTTGCAAGAGCTCAACAGTAGTGAGCGCAATATTTTAACCGTAGAAGATC
CGATCGAATTTGATATTGATGGTATCGGTCAGACCCAAGTCAACCCAAGGGTAGACATGACCTTCGCGCGGGGGCTACGC
GCTATTTTGCGTCAAGACCCCGATGTGGTGATGGTGGGGGAAATTCGTGATTTGGAAACGGCGCAAATTGCAGTGCAAGC
CTCGCTGACCGGTCACTTAGTGATGTCGACTCTGCACACCAATACGGCCGTTGGGGCGGTGACTCGGCTGCGCGATATGG
GCATCGAGCCTTTTTTGATCTCCTCTTCACTGCTGGGTGTTCTGGCTCAGCGTTTGGTGCGCACCTTATGCCCAGATTGC
AAAGAGCCTTACGAGGCGGACAAAGAGCAGCGCAAACTGTTTGATAGTAAGAAAAAAGAACCGCTGATCCTTTATCGAGC
CACGGGCTGCCCTAAATGTAACCACAAAGGTTACCGTGGCCGAACCGGTATCCACGAGCTGCTGCTGGTGGATGACGCGT
TGCAGGAGCTGATCCATAGCGAGGCGGGCGAACAGGCGATGGAGAAACACATTCGCGCGACCACGCCGAGCATTCGTGAT
GATGGTCTAGATAAGGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATGCGGGTGACTAAGGAGTCCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1P9R
  PDB 1P9W
  PDB 2BH1
  PDB 4KSR
  PDB 4KSS

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.412

100

0.431
  pilB Legionella pneumophila strain ERS1305867

47.126

86.481

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

44.619

88.668

0.396
  pilB Vibrio campbellii strain DS40M4

44.118

87.873

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.745

77.932

0.388
  pilB Vibrio cholerae strain A1552

48.485

78.728

0.382
  pilF Thermus thermophilus HB27

47.761

79.92

0.382
  pilB Acinetobacter baumannii D1279779

46.21

81.312

0.376

Multiple sequence alignment