Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SaO322_RS07725 Genome accession   NZ_CP038460
Coordinates   1549463..1549960 (-) Length   165 a.a.
NCBI ID   WP_078061106.1    Uniprot ID   -
Organism   Staphylococcus aureus strain B119     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1544463..1554960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SaO322_RS07700 (SaO322_01421) gcvPB 1544626..1546098 (-) 1473 WP_000202177.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  SaO322_RS07705 (SaO322_01422) gcvPA 1546091..1547437 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SaO322_RS07710 (SaO322_01423) gcvT 1547457..1548548 (-) 1092 WP_000093343.1 glycine cleavage system aminomethyltransferase GcvT -
  SaO322_RS07715 (SaO322_01424) - 1548706..1549230 (-) 525 WP_001015120.1 shikimate kinase -
  SaO322_RS07720 - 1549220..1549366 (-) 147 WP_001789879.1 hypothetical protein -
  SaO322_RS07725 (SaO322_01425) comGF 1549463..1549960 (-) 498 WP_078061106.1 competence type IV pilus minor pilin ComGF Machinery gene
  SaO322_RS07730 (SaO322_01426) comGE 1549878..1550177 (-) 300 WP_000844409.1 hypothetical protein Machinery gene
  SaO322_RS07735 (SaO322_01427) comGD 1550164..1550610 (-) 447 WP_118848117.1 competence type IV pilus minor pilin ComGD Machinery gene
  SaO322_RS07740 (SaO322_01428) comGC 1550588..1550899 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SaO322_RS07745 (SaO322_01429) comGB 1550913..1551983 (-) 1071 WP_000776418.1 competence type IV pilus assembly protein ComGB Machinery gene
  SaO322_RS07750 (SaO322_01430) comGA 1551955..1552929 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SaO322_RS07755 (SaO322_01431) - 1552981..1553604 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SaO322_RS07760 (SaO322_01432) - 1553601..1553930 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SaO322_RS07765 (SaO322_01433) - 1553930..1554916 (-) 987 WP_118848118.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19167.92 Da        Isoelectric Point: 10.2192

>NTDB_id=354530 SaO322_RS07725 WP_078061106.1 1549463..1549960(-) (comGF) [Staphylococcus aureus strain B119]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMLNNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYA

Nucleotide


Download         Length: 498 bp        

>NTDB_id=354530 SaO322_RS07725 WP_078061106.1 1549463..1549960(-) (comGF) [Staphylococcus aureus strain B119]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAACAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGCTTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGCATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(69-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.171

99.394

0.976
  comGF Staphylococcus aureus MW2

96.951

99.394

0.964

Multiple sequence alignment