Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SaO322_RS07750 Genome accession   NZ_CP038460
Coordinates   1551955..1552929 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain B119     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1546955..1557929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SaO322_RS07710 (SaO322_01423) gcvT 1547457..1548548 (-) 1092 WP_000093343.1 glycine cleavage system aminomethyltransferase GcvT -
  SaO322_RS07715 (SaO322_01424) - 1548706..1549230 (-) 525 WP_001015120.1 shikimate kinase -
  SaO322_RS07720 - 1549220..1549366 (-) 147 WP_001789879.1 hypothetical protein -
  SaO322_RS07725 (SaO322_01425) comGF 1549463..1549960 (-) 498 WP_078061106.1 competence type IV pilus minor pilin ComGF Machinery gene
  SaO322_RS07730 (SaO322_01426) comGE 1549878..1550177 (-) 300 WP_000844409.1 hypothetical protein Machinery gene
  SaO322_RS07735 (SaO322_01427) comGD 1550164..1550610 (-) 447 WP_118848117.1 competence type IV pilus minor pilin ComGD Machinery gene
  SaO322_RS07740 (SaO322_01428) comGC 1550588..1550899 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SaO322_RS07745 (SaO322_01429) comGB 1550913..1551983 (-) 1071 WP_000776418.1 competence type IV pilus assembly protein ComGB Machinery gene
  SaO322_RS07750 (SaO322_01430) comGA 1551955..1552929 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SaO322_RS07755 (SaO322_01431) - 1552981..1553604 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SaO322_RS07760 (SaO322_01432) - 1553601..1553930 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SaO322_RS07765 (SaO322_01433) - 1553930..1554916 (-) 987 WP_118848118.1 ROK family glucokinase -
  SaO322_RS07770 - 1554913..1555116 (-) 204 WP_000087561.1 YqgQ family protein -
  SaO322_RS07775 (SaO322_01434) - 1555097..1556560 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  SaO322_RS07780 (SaO322_01435) - 1556572..1557111 (-) 540 WP_000164552.1 5-formyltetrahydrofolate cyclo-ligase -
  SaO322_RS07785 (SaO322_01436) rpmG 1557320..1557469 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=354535 SaO322_RS07750 WP_000697220.1 1551955..1552929(-) (comGA) [Staphylococcus aureus strain B119]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=354535 SaO322_RS07750 WP_000697220.1 1551955..1552929(-) (comGA) [Staphylococcus aureus strain B119]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCTACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAAGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment