Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EBI04_RS07265 Genome accession   NZ_CP038452
Coordinates   1414323..1416593 (+) Length   756 a.a.
NCBI ID   WP_135256903.1    Uniprot ID   -
Organism   Thermus caldilimi strain YIM 78456     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1409323..1421593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBI04_RS07240 - 1409793..1410797 (+) 1005 WP_135256899.1 homoisocitrate dehydrogenase -
  EBI04_RS07245 pilM 1410985..1412117 (+) 1133 Protein_1446 type IV pilus assembly protein PilM -
  EBI04_RS07250 pilN 1412110..1412733 (+) 624 WP_135256900.1 flagellar protein FliT Machinery gene
  EBI04_RS07255 pilO 1412723..1413346 (+) 624 WP_135256901.1 type 4a pilus biogenesis protein PilO Machinery gene
  EBI04_RS07260 - 1413343..1414311 (+) 969 WP_135256902.1 competence protein -
  EBI04_RS07265 pilQ 1414323..1416593 (+) 2271 WP_135256903.1 secretin N-terminal domain-containing protein Machinery gene
  EBI04_RS07270 aroC 1416696..1417847 (+) 1152 WP_135256904.1 chorismate synthase -
  EBI04_RS07275 - 1417863..1418417 (+) 555 WP_135256905.1 shikimate kinase -
  EBI04_RS07280 - 1418395..1419444 (+) 1050 WP_135256906.1 3-dehydroquinate synthase family protein -
  EBI04_RS07285 rsmF 1419491..1420851 (-) 1361 Protein_1454 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF -
  EBI04_RS13815 - 1420909..1421034 (+) 126 WP_276605478.1 hypothetical protein -
  EBI04_RS07290 acpS 1421031..1421408 (+) 378 WP_135256907.1 holo-ACP synthase -

Sequence

Protein


Download         Length: 756 a.a.        Molecular weight: 82694.12 Da        Isoelectric Point: 5.5676

>NTDB_id=354479 EBI04_RS07265 WP_135256903.1 1414323..1416593(+) (pilQ) [Thermus caldilimi strain YIM 78456]
MKLMIPGLLILGMGALAGSLPQEPRFDAKVDLKVSESQVRAGFTLPLDVVLEALARSVGLQPLIYRAYDASGDPAKAQPP
LPNIKLDFQGKPFREVWDLLFATYGTQFNLDYLLLPPDVVVVAPTQVITALVDAPSRTGAMERKPYLVAIPEIAYRRTET
DAQGQARTVVNIDGAKGWVQNDLLPFLSREASGLSVNWIVVEEGGKLRALLSVLATPEQHVRFSDILQRAGIDFRPLPAL
TLPKPKVERSYILTHTTFPEVLSFLQTQVPNAQVAVVPTDPKRALITATEEDHARIAELLKVADVPKPTPVVRRVYTLQN
LTFQEAQERLKPVLERELKGARLEGVPGNPKALLLEATEADQAFFAEVLKAADVPPQVAPPTQEAMVRRLYPLRFADAEK
VAPFLAREVPGIVVQTVPGQPVLSVRGTEKQLSEVENLLAQIDRAPEQGPPVFQRSYQLSNAKAADLAKVLQEALQARQA
QAPQGQVQPQAPVRQATVVADERTNTLIVTGTQEDLSLVEGLIPRLDQAVPQVNLRVRIQEVQSNFTRNLGLKWNTIAGG
NVAASVLDSGLSLIFDSTRSLAALNILATLEALQRQGLSRALRDVNQTVLNNQTARLQSGETFLIRRINPSTGAVERVPF
DIGLIVEVTPQITADGQILLNIKAEVSGNVQRNPVDGDVDRFTKQVVTTTLRVRDGQTVVLGGLTSQENNQVQQGVPLLM
DIPLIGELFKQRTQETTDRELLVVITADILKETASR

Nucleotide


Download         Length: 2271 bp        

>NTDB_id=354479 EBI04_RS07265 WP_135256903.1 1414323..1416593(+) (pilQ) [Thermus caldilimi strain YIM 78456]
ATGAAACTTATGATCCCCGGCCTTTTGATCCTGGGCATGGGGGCTCTGGCGGGAAGCCTTCCCCAGGAACCCCGCTTTGA
CGCCAAGGTGGACCTGAAGGTATCCGAAAGCCAGGTGCGGGCCGGGTTTACCCTGCCCCTGGACGTGGTCCTGGAAGCCC
TGGCCCGGAGCGTGGGCCTTCAGCCCCTTATCTACCGGGCCTACGATGCCTCCGGCGACCCAGCCAAGGCCCAGCCCCCT
TTACCCAACATCAAGCTGGACTTCCAGGGCAAACCCTTCCGGGAGGTCTGGGACCTCCTCTTCGCCACTTACGGCACCCA
GTTCAACCTGGACTACCTCCTCCTGCCTCCCGACGTGGTGGTGGTGGCCCCCACCCAGGTGATCACCGCCTTGGTAGACG
CTCCAAGCCGCACCGGGGCCATGGAGCGGAAGCCCTACCTGGTGGCCATCCCCGAGATCGCCTACCGGCGCACGGAAACC
GACGCCCAGGGCCAAGCCCGCACCGTGGTGAACATAGACGGGGCCAAGGGCTGGGTGCAAAACGATCTCCTGCCCTTCCT
TTCCCGGGAAGCCAGCGGGCTTAGCGTGAACTGGATTGTCGTAGAGGAAGGGGGCAAACTCAGGGCCCTACTCTCTGTCC
TGGCCACCCCAGAACAGCACGTTCGCTTCTCGGACATCCTTCAGCGGGCTGGGATTGACTTCCGCCCCCTACCCGCCCTC
ACCTTACCCAAACCAAAGGTGGAGCGGAGCTATATCCTCACCCACACCACCTTCCCCGAGGTGCTTTCCTTTCTGCAAAC
CCAGGTTCCGAACGCCCAGGTGGCCGTGGTTCCCACCGACCCCAAACGGGCCCTCATCACCGCCACCGAGGAGGACCACG
CCCGCATCGCCGAACTCCTAAAGGTGGCCGATGTGCCCAAGCCCACCCCCGTGGTGCGCCGGGTCTACACCTTGCAAAAC
CTCACCTTCCAGGAAGCCCAGGAAAGGCTTAAACCCGTCCTGGAAAGGGAGTTGAAAGGGGCCCGCCTGGAAGGTGTCCC
GGGTAACCCCAAAGCCCTTCTTCTGGAGGCTACCGAAGCCGACCAGGCCTTCTTCGCCGAGGTCCTCAAGGCTGCCGATG
TGCCTCCCCAGGTGGCCCCGCCCACCCAGGAGGCCATGGTGCGCAGGCTCTACCCCTTGCGCTTCGCCGATGCTGAAAAG
GTAGCTCCCTTCCTGGCCCGGGAGGTGCCGGGAATTGTGGTGCAGACGGTGCCCGGGCAACCCGTCCTCTCCGTGCGGGG
AACGGAGAAACAGCTTTCGGAAGTAGAGAACCTCCTGGCCCAGATCGACCGGGCTCCCGAGCAGGGGCCACCTGTCTTCC
AGCGCTCCTACCAGCTCTCCAACGCCAAAGCCGCCGACCTGGCCAAGGTGCTCCAGGAGGCCTTGCAAGCCCGGCAGGCC
CAGGCTCCCCAGGGCCAGGTCCAGCCCCAAGCCCCCGTCCGCCAGGCCACGGTGGTGGCGGACGAGCGCACCAACACCCT
GATCGTCACCGGTACCCAGGAGGACTTGAGCCTGGTGGAGGGCCTCATCCCCAGGCTGGACCAGGCGGTGCCCCAGGTGA
ACCTGAGGGTGCGGATCCAGGAGGTGCAGTCCAACTTCACCCGCAACCTGGGCCTCAAGTGGAACACCATCGCCGGGGGG
AACGTGGCGGCCAGCGTCCTGGATTCGGGGCTTTCCCTCATCTTCGACAGCACCCGTAGCCTTGCCGCCCTGAACATCCT
GGCCACCCTCGAGGCCCTCCAGCGCCAGGGCCTTTCCCGAGCCCTTAGGGATGTGAACCAAACCGTGCTCAACAACCAGA
CTGCCCGCCTCCAGTCCGGGGAAACCTTCCTCATCCGGCGCATCAACCCCTCCACCGGGGCGGTGGAGCGGGTGCCCTTC
GACATCGGCCTCATCGTGGAGGTGACCCCCCAGATCACCGCCGACGGGCAGATCCTCCTCAACATCAAGGCGGAGGTTTC
CGGCAACGTCCAGCGCAACCCCGTGGACGGGGACGTGGACCGCTTCACCAAGCAGGTGGTGACCACCACCCTGAGGGTGC
GGGACGGCCAGACCGTGGTCCTGGGGGGCCTCACCTCCCAGGAAAACAACCAGGTGCAACAGGGAGTCCCCCTCCTCATG
GACATCCCCTTGATCGGGGAACTCTTCAAACAACGCACCCAGGAAACTACCGACCGGGAGCTTCTGGTGGTCATCACCGC
CGACATCTTGAAGGAAACCGCAAGCCGCTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(592-751)

Bacterial type II/III secretion system short domain (Secretin_N)

(389-448)

Bacterial type II/III secretion system short domain (Secretin_N)

(455-532)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Thermus thermophilus HB27

82.685

98.545

0.815

Multiple sequence alignment