Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GPW70_RS05890 Genome accession   NZ_CP046740
Coordinates   201819..203330 (+) Length   503 a.a.
NCBI ID   WP_000138174.1    Uniprot ID   P37093
Organism   Vibrio cholerae strain 3566-06     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196819..208330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW70_RS05870 hslO 197316..198191 (-) 876 WP_032467814.1 Hsp33 family molecular chaperone HslO -
  GPW70_RS05875 hslR 198225..198602 (-) 378 WP_000098851.1 ribosome-associated heat shock protein Hsp15 -
  GPW70_RS05880 gspC 198832..199749 (+) 918 WP_001887342.1 type II secretion system protein GspC -
  GPW70_RS05885 gspD 199795..201822 (+) 2028 WP_001887341.1 type II secretion system secretin GspD -
  GPW70_RS05890 pilB/pilB1 201819..203330 (+) 1512 WP_000138174.1 type II secretion system ATPase GspE Machinery gene
  GPW70_RS05895 gspF 203330..204550 (+) 1221 WP_000718695.1 type II secretion system inner membrane protein GspF -
  GPW70_RS05900 gspG 204591..205031 (+) 441 WP_000738789.1 type II secretion system major pseudopilin GspG -
  GPW70_RS05905 gspH 205065..205649 (+) 585 WP_032473020.1 type II secretion system minor pseudopilin GspH -
  GPW70_RS05910 gspI 205639..205992 (+) 354 WP_000837699.1 type II secretion system minor pseudopilin GspI -
  GPW70_RS05915 gspJ 205979..206647 (+) 669 WP_032473019.1 type II secretion system minor pseudopilin GspJ -
  GPW70_RS05920 gspK 206637..207647 (+) 1011 WP_001887340.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 56358.58 Da        Isoelectric Point: 6.2107

>NTDB_id=354478 GPW70_RS05890 WP_000138174.1 201819..203330(+) (pilB/pilB1) [Vibrio cholerae strain 3566-06]
MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVY
QRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGV
LREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLG
MTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLR
AILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDC
KEPYEADKEQRKLFDSKKKEPLILYRATGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRD
DGLDKVRQGITSLEEVMRVTKES

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=354478 GPW70_RS05890 WP_000138174.1 201819..203330(+) (pilB/pilB1) [Vibrio cholerae strain 3566-06]
ATGACCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTT
GGTGCTGGATTGGAATGAGGATTTCTCCCAAGCCAGCATTTATTACTTAGCGCCGTTGTCGATGGAGGCGCTCGTCGAAA
CCAAGCGGGTGGTCAAGCACGCTTTCCAACTGATTGAGCTCTCTCAAGCGGAGTTTGAAAGCAAGCTAACCCAAGTCTAT
CAGCGTGACTCTTCAGAAGCGCGTCAATTGATGGAAGACATTGGTGCCGACAGTGATGACTTCTTCTCACTGGCGGAAGA
GCTGCCTCAAAACGAAGATTTGCTAGAAAGTGAAGATGATGCGCCGATCATCAAACTGATCAACGCCATGTTGGGCGAAG
CGATCAAAGAAGGCGCGTCAGATATTCACATTGAAACTTTTGAAAAAACGCTGTCGATCCGTTTTCGAGTTGATGGCGTG
CTGCGCGAAGTACTGGCACCCAGCCGCAAACTCTCATCGCTGCTCGTTTCACGGGTCAAAGTGATGGCCAAGCTCGACAT
TGCCGAAAAACGAGTGCCACAAGATGGCCGTATTTCGCTGCGTATCGGTGGCCGCGCGGTCGATGTGCGGGTTTCAACCA
TGCCTTCATCACATGGTGAGCGAGTGGTAATGCGTTTGCTGGATAAAAACGCGACGCGCCTTGATCTGCACAGTTTGGGT
ATGACGGCGCATAACCATGATAACTTCCGCCGTTTGATTAAGCGGCCGCATGGGATCATTCTGGTGACTGGCCCAACCGG
CTCGGGTAAATCGACTACCTTGTACGCGGGTTTGCAAGAGCTCAACAGCAGCGAGCGCAACATTTTAACCGTAGAAGATC
CGATCGAATTTGATATTGATGGTATCGGTCAGACCCAAGTCAACCCAAGGGTAGACATGACCTTCGCGCGGGGGCTACGC
GCTATTTTGCGTCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGTGACTTGGAAACGGCGCAAATTGCAGTGCAAGC
CTCGCTGACCGGTCACTTAGTGATGTCGACTCTGCACACCAACACGGCCGTTGGGGCGGTGACTCGGCTGCGCGATATGG
GCATCGAGCCTTTTTTGATCTCTTCTTCATTGCTCGGTGTTCTGGCTCAGCGTTTGGTGCGCACCTTATGCCCAGATTGT
AAAGAGCCTTACGAGGCGGACAAAGAGCAGCGCAAACTGTTTGATAGCAAGAAAAAAGAGCCACTAATTCTTTATCGTGC
AACGGGCTGCCCTAAATGTAACCACAAAGGTTACCGTGGCCGAACCGGTATCCACGAGCTGCTGCTGGTGGATGACGCGT
TGCAGGAGCTGATCCATAGCGAGGCGGGCGAACAGGCGATGGAGAAACACATTCGCGCGACCACGCCGAGCATTCGTGAT
GATGGTCTAGATAAGGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATGCGGGTGACTAAGGAGTCCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1P9R
  PDB 1P9W
  PDB 2BH1
  PDB 4KSR
  PDB 4KSS

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.412

100

0.431
  pilB Legionella pneumophila strain ERS1305867

47.126

86.481

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

44.619

88.668

0.396
  pilB Vibrio campbellii strain DS40M4

44.118

87.873

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.745

77.932

0.388
  pilB Vibrio cholerae strain A1552

48.485

78.728

0.382
  pilF Thermus thermophilus HB27

47.761

79.92

0.382
  pilB Acinetobacter baumannii D1279779

46.21

81.312

0.376

Multiple sequence alignment