Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   E4187_RS01870 Genome accession   NZ_CP038445
Coordinates   404979..405554 (-) Length   191 a.a.
NCBI ID   WP_005328769.1    Uniprot ID   -
Organism   Aeromonas media strain R25-3     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 371667..405554 404979..405554 within 0


Gene organization within MGE regions


Location: 371667..405554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4187_RS01715 (E4187_01745) cspA 371667..371879 (-) 213 WP_025328559.1 RNA chaperone/antiterminator CspA -
  E4187_RS01720 (E4187_01750) yjbD 372273..372557 (-) 285 WP_025328558.1 DUF3811 domain-containing protein -
  E4187_RS23065 (E4187_01755) - 372898..373020 (+) 123 WP_080675244.1 hypothetical protein -
  E4187_RS01725 (E4187_01760) - 373069..373839 (-) 771 WP_005328770.1 transporter substrate-binding domain-containing protein -
  E4187_RS23175 - 374321..374443 (+) 123 Protein_329 hypothetical protein -
  E4187_RS23180 (E4187_01765) - 374528..375274 (+) 747 WP_113725460.1 DUF4113 domain-containing protein -
  E4187_RS01735 (E4187_01770) - 375383..375691 (-) 309 WP_047440196.1 helix-turn-helix transcriptional regulator -
  E4187_RS01740 (E4187_01775) - 375790..376260 (+) 471 WP_017780886.1 hypothetical protein -
  E4187_RS01745 (E4187_01780) - 376470..376661 (+) 192 WP_017780887.1 hypothetical protein -
  E4187_RS01750 (E4187_01785) tetR 376993..377628 (-) 636 WP_017780888.1 tetracycline resistance transcriptional repressor TetR -
  E4187_RS01755 (E4187_01790) tet(E) 377709..378926 (+) 1218 WP_017780889.1 tetracycline efflux MFS transporter Tet(E) -
  E4187_RS01760 (E4187_01795) - 379014..379682 (-) 669 WP_017780890.1 hypothetical protein -
  E4187_RS22815 (E4187_01800) - 379841..380002 (-) 162 Protein_337 IS5/IS1182 family transposase -
  E4187_RS01765 (E4187_01805) - 380220..382382 (-) 2163 WP_171269529.1 AAA family ATPase -
  E4187_RS01770 (E4187_01810) - 382490..383658 (+) 1169 WP_085943968.1 IS3-like element ISAeme20 family transposase -
  E4187_RS01775 (E4187_01815) - 383679..384083 (-) 405 WP_171269530.1 VPA1267 family protein -
  E4187_RS01780 (E4187_01820) - 384083..384562 (-) 480 WP_049866848.1 hypothetical protein -
  E4187_RS01785 (E4187_01825) - 384550..386427 (-) 1878 WP_041207315.1 integrase -
  E4187_RS01790 (E4187_01830) - 386437..388005 (-) 1569 WP_171269322.1 site-specific integrase -
  E4187_RS01795 (E4187_01835) - 388002..389570 (-) 1569 WP_216370113.1 site-specific integrase -
  E4187_RS23185 - 389814..389936 (+) 123 Protein_345 hypothetical protein -
  E4187_RS23190 (E4187_01840) - 390021..390767 (+) 747 WP_365843053.1 DUF4113 domain-containing protein -
  E4187_RS01805 (E4187_01845) - 390876..391196 (-) 321 WP_171269969.1 helix-turn-helix transcriptional regulator -
  E4187_RS01810 (E4187_01850) - 391284..391724 (+) 441 WP_171269531.1 hypothetical protein -
  E4187_RS01815 (E4187_01855) - 391962..392153 (+) 192 WP_017780887.1 hypothetical protein -
  E4187_RS01820 (E4187_01860) tetR 392485..393120 (-) 636 WP_017780888.1 tetracycline resistance transcriptional repressor TetR -
  E4187_RS01825 (E4187_01865) tet(E) 393200..394417 (+) 1218 WP_017780889.1 tetracycline efflux MFS transporter Tet(E) -
  E4187_RS01830 (E4187_01870) - 394505..395173 (-) 669 WP_017780890.1 hypothetical protein -
  E4187_RS22820 (E4187_01875) - 395332..395493 (-) 162 Protein_353 IS5/IS1182 family transposase -
  E4187_RS22690 - 395711..396214 (-) 504 WP_216370115.1 ATP-dependent RecD-like DNA helicase -
  E4187_RS01835 (E4187_01880) - 396229..397872 (-) 1644 WP_216370116.1 AAA family ATPase -
  E4187_RS01840 (E4187_01885) - 397980..399148 (+) 1169 WP_085943968.1 IS3-like element ISAeme20 family transposase -
  E4187_RS01845 (E4187_01890) - 399169..399573 (-) 405 WP_171269530.1 VPA1267 family protein -
  E4187_RS01850 (E4187_01895) - 399573..400052 (-) 480 WP_049866848.1 hypothetical protein -
  E4187_RS01855 (E4187_01900) - 400040..401917 (-) 1878 WP_041207315.1 integrase -
  E4187_RS01860 (E4187_01905) - 401927..403495 (-) 1569 WP_171269322.1 site-specific integrase -
  E4187_RS01865 (E4187_01910) - 403492..404814 (-) 1323 WP_041207317.1 site-specific integrase -
  E4187_RS01870 (E4187_01915) ssb 404979..405554 (-) 576 WP_005328769.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 191 a.a.        Molecular weight: 20953.37 Da        Isoelectric Point: 5.9247

>NTDB_id=354403 E4187_RS01870 WP_005328769.1 404979..405554(-) (ssb) [Aeromonas media strain R25-3]
MASRGINKVILIGNLGQDPEVRYMPSGGAVTNITLATSDTWRDKQTGEQKERTEWHRVVFMGKLAEVAGEYLKKGSQVYV
EGKLQTRKWQDQSGQERYTTEVLVDSFTGVMQMLGGRPQGGAGQGMGGQQSQGNWGQPQQAAPQQAMQRPAAAPQQNMQP
QGGYGRPAQQPQSAPPVYNEPPMDFDDDIPF

Nucleotide


Download         Length: 576 bp        

>NTDB_id=354403 E4187_RS01870 WP_005328769.1 404979..405554(-) (ssb) [Aeromonas media strain R25-3]
ATGGCCAGTCGAGGCATCAACAAAGTCATTCTGATCGGTAACCTGGGGCAAGACCCGGAAGTGCGCTACATGCCGAGCGG
CGGTGCCGTGACTAACATCACCCTGGCCACCTCCGACACCTGGCGCGACAAGCAGACCGGTGAGCAGAAAGAGCGAACCG
AGTGGCACCGCGTGGTGTTCATGGGCAAGCTGGCCGAAGTGGCCGGCGAGTACCTGAAGAAGGGTTCCCAGGTCTATGTC
GAAGGCAAGCTGCAGACCCGCAAGTGGCAGGATCAGAGTGGCCAGGAGCGTTACACCACAGAGGTGCTGGTCGACAGCTT
CACCGGCGTAATGCAGATGCTGGGCGGCCGTCCGCAAGGCGGCGCAGGCCAGGGCATGGGTGGTCAACAGTCCCAGGGCA
ACTGGGGTCAGCCACAACAGGCCGCCCCCCAGCAAGCGATGCAACGCCCGGCCGCCGCCCCGCAGCAGAATATGCAGCCG
CAGGGTGGTTACGGTCGTCCTGCCCAGCAGCCTCAGTCTGCACCGCCGGTCTACAACGAGCCGCCGATGGATTTCGACGA
CGATATCCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

69.744

100

0.712

  ssb Glaesserella parasuis strain SC1401

53.968

98.953

0.534

  ssb Neisseria meningitidis MC58

46.809

98.429

0.461

  ssb Neisseria gonorrhoeae MS11

46.237

97.382

0.45


Multiple sequence alignment