Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   GN312_RS25415 Genome accession   NZ_CP046527
Coordinates   5059582..5060592 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain RM19259     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5054582..5065592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN312_RS25395 (GN312_25395) mepM 5055450..5056772 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  GN312_RS25400 (GN312_25400) znuA 5056788..5057720 (-) 933 WP_001303192.1 zinc ABC transporter substrate-binding protein ZnuA -
  GN312_RS25405 (GN312_25405) znuC 5057799..5058554 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  GN312_RS25410 (GN312_25410) znuB 5058551..5059336 (+) 786 WP_000571476.1 zinc ABC transporter permease subunit ZnuB -
  GN312_RS25415 (GN312_25415) ruvB 5059582..5060592 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  GN312_RS25420 (GN312_25420) ruvA 5060601..5061212 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  GN312_RS25425 (GN312_25425) yobI 5061351..5061416 (-) 66 WP_010723105.1 stress response small protein YobI -
  GN312_RS25430 (GN312_25430) yebB 5061487..5062089 (+) 603 WP_001024949.1 YebB family permuted papain-like enzyme -
  GN312_RS25435 (GN312_25435) ruvC 5062091..5062612 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  GN312_RS25440 (GN312_25440) yebC 5062647..5063387 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  GN312_RS25445 (GN312_25445) nudB 5063416..5063868 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=353050 GN312_RS25415 WP_000568519.1 5059582..5060592(-) (ruvB) [Escherichia coli strain RM19259]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=353050 GN312_RS25415 WP_000568519.1 5059582..5060592(-) (ruvB) [Escherichia coli strain RM19259]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment