Detailed information NTG0352882

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E4K03_RS15880
• Genome accession: NZ_CP038262
• Coordinates: 3250747..3251865 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain LEV1449/17Ec
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3245747..3256865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4K03_RS15870 (E4K03_15870)	-	3248863..3249555 (-)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-
E4K03_RS15875 (E4K03_15875)	pilT	3249682..3250719 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
E4K03_RS15880 (E4K03_15880)	pilU	3250747..3251865 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E4K03_RS15885 (E4K03_15885)	fur	3251935..3252372 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
E4K03_RS15890 (E4K03_15890)	-	3252484..3252882 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
E4K03_RS15895 (E4K03_15895)	-	3252937..3253260 (-)	324	WP_000442589.1	RnfH family protein	-
E4K03_RS15900 (E4K03_15900)	-	3253260..3254327 (-)	1068	WP_004835750.1	hypothetical protein	-
E4K03_RS15910 (E4K03_15910)	-	3254480..3254935 (-)	456	WP_000782976.1	bacteriohemerythrin	-
E4K03_RS15915 (E4K03_15915)	-	3255188..3256036 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=352882 E4K03_RS15880 WP_000347036.1 3250747..3251865(+) (pilU) [Acinetobacter baumannii strain LEV1449/17Ec]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=352882 E4K03_RS15880 WP_000347036.1 3250747..3251865(+) (pilU) [Acinetobacter baumannii strain LEV1449/17Ec]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392