Detailed information NTG0352803

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E4K02_RS00505
• Genome accession: NZ_CP038258
• Coordinates: 87503..88621 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 39741
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 82503..93621

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E4K02_RS00460 (E4K02_00460)	-	82800..83090 (+)	291	Protein_87	class II glutamine amidotransferase	-
E4K02_RS00465 (E4K02_00465)	-	83343..83657 (+)	315	WP_171072075.1	bacteriohemerythrin	-
E4K02_RS00470 (E4K02_00470)	-	83592..84560 (-)	969	WP_014538385.1	IS30 family transposase	-
E4K02_RS00475 (E4K02_00475)	-	84673..84888 (+)	216	Protein_90	hemerythrin	-
E4K02_RS00485 (E4K02_00485)	-	85041..86108 (+)	1068	WP_004835750.1	hypothetical protein	-
E4K02_RS00490 (E4K02_00490)	-	86108..86431 (+)	324	WP_000442589.1	RnfH family protein	-
E4K02_RS00495 (E4K02_00495)	-	86486..86884 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
E4K02_RS00500 (E4K02_00500)	fur	86996..87433 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
E4K02_RS00505 (E4K02_00505)	pilU	87503..88621 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E4K02_RS00510 (E4K02_00510)	pilT	88649..89686 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
E4K02_RS00515 (E4K02_00515)	-	89813..90505 (+)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=352803 E4K02_RS00505 WP_000347036.1 87503..88621(-) (pilU) [Acinetobacter baumannii strain 39741]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=352803 E4K02_RS00505 WP_000347036.1 87503..88621(-) (pilU) [Acinetobacter baumannii strain 39741]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392