Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   E1742_RS18930 Genome accession   NZ_CP038026
Coordinates   4298086..4299555 (+) Length   489 a.a.
NCBI ID   WP_134386638.1    Uniprot ID   -
Organism   Pseudoduganella plicata strain DSM 17505     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4293086..4304555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1742_RS18905 (E1742_18905) - 4294542..4295564 (+) 1023 WP_371860167.1 SIS domain-containing protein -
  E1742_RS18910 (E1742_18910) - 4295564..4296118 (+) 555 WP_229466084.1 HAD family hydrolase -
  E1742_RS18915 (E1742_18915) lptC 4296127..4296729 (+) 603 WP_134386633.1 LPS export ABC transporter periplasmic protein LptC -
  E1742_RS18920 (E1742_18920) lptA 4296740..4297315 (+) 576 WP_134386635.1 lipopolysaccharide transport periplasmic protein LptA -
  E1742_RS18925 (E1742_18925) lptB 4297315..4298079 (+) 765 WP_134386637.1 LPS export ABC transporter ATP-binding protein -
  E1742_RS18930 (E1742_18930) rpoN/rpoN1 4298086..4299555 (+) 1470 WP_134386638.1 RNA polymerase factor sigma-54 Machinery gene
  E1742_RS18935 (E1742_18935) hpf 4299640..4299990 (+) 351 WP_134386640.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  E1742_RS18940 (E1742_18940) - 4300246..4301327 (+) 1082 Protein_3776 enoyl-CoA hydratase/isomerase family protein -
  E1742_RS18945 (E1742_18945) - 4301342..4302394 (+) 1053 WP_134386642.1 amidohydrolase family protein -
  E1742_RS18950 (E1742_18950) rapZ 4302433..4303296 (-) 864 WP_134386644.1 RNase adapter RapZ -
  E1742_RS18955 (E1742_18955) - 4303351..4303590 (-) 240 WP_134386646.1 hypothetical protein -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 54348.90 Da        Isoelectric Point: 5.1107

>NTDB_id=351535 E1742_RS18930 WP_134386638.1 4298086..4299555(+) (rpoN/rpoN1) [Pseudoduganella plicata strain DSM 17505]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSLRLLADGAIGQQSAPEAPAEGPP
QQTDAAAPAEAEPYDGADADRGPEGDTDWTDAGRGKAPDDDDARPQLEASHCSLREHLMEQMRVTVQQPRDRALVELIID
ALDENGYLEESLDEIHGRLPEELEVEMEELRTALALLQSFDPVGVGARSASECLALQIRKMPGVPLVTRRMALCIVEKHL
AWFAQRDFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAYASDVSDYVVPDVIVKKGRNGWVVTLNNDVMPRLRVNALYA
SLLKQGKGESQMGAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVTTQ
KYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQFTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREALKI
PPVSLRKSL

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=351535 E1742_RS18930 WP_134386638.1 4298086..4299555(+) (rpoN/rpoN1) [Pseudoduganella plicata strain DSM 17505]
ATGAAACAGTCTCTGCAACTGCGAACGTCGCAGCACCTGGCGCTGACACCACAGCTGCAGCAGTCCATCCGGCTGCTGCA
GTTGTCCACGCTGGAACTGCACCAGGAACTGGAACAGCTGCTGACGGATAACCCGCTGCTGGAACGGCTCGACGATCCGC
TCGACCGTTCGCTGCGGCTGCTGGCCGATGGCGCCATCGGCCAGCAGAGCGCGCCCGAGGCACCGGCCGAAGGCCCGCCC
CAGCAAACCGACGCGGCGGCCCCCGCAGAGGCAGAACCGTATGACGGCGCCGACGCGGACCGCGGCCCGGAAGGCGATAC
CGACTGGACCGACGCGGGCCGCGGCAAGGCGCCGGACGACGACGACGCGCGGCCGCAACTGGAAGCGAGCCATTGTTCGC
TGCGCGAACACCTGATGGAGCAGATGCGGGTGACGGTGCAACAGCCGCGCGACCGGGCGCTTGTCGAGCTGATCATCGAC
GCGCTGGACGAAAACGGCTACCTGGAAGAGAGCCTGGACGAGATCCACGGCCGCCTGCCCGAAGAGCTGGAAGTGGAAAT
GGAAGAGCTGCGCACGGCGCTGGCGCTGCTGCAGAGCTTCGATCCGGTGGGCGTCGGCGCCCGCAGCGCGTCCGAATGCC
TGGCGCTGCAGATCCGCAAGATGCCGGGCGTGCCGCTGGTGACGCGCCGGATGGCGCTGTGCATTGTCGAGAAACACCTG
GCCTGGTTTGCCCAGCGCGACTTCAACAAGCTCAAGAAGGCGCTCGATTGCGACGACGAAGACCTGCGCGAAGCACAGGC
CGTCATTCGCCAGTGCAACCCCCATCCGGGCGCCGCGTATGCGTCCGACGTGTCAGACTATGTCGTACCGGATGTGATTG
TGAAGAAGGGCCGCAACGGCTGGGTGGTCACGCTGAACAACGACGTGATGCCGCGCCTGCGCGTGAACGCGCTGTATGCC
AGCCTGCTCAAACAGGGCAAGGGCGAAAGCCAGATGGGCGCGCAGCTGCAGGAAGCGAAGTGGCTGATCAAGAATATGCG
CCAGCGCTTCGACACGATCCTGCGCGTCGCACAGGCCATCGTCGAGCGGCAGAAGAACTTTTTCTCGCATGGCGCGGTGG
CCATGCGACCCCTTGTGTTGCGTGAAATAGCTGATACACTGGGGCTACACGAGAGTACGATCTCTCGGGTGACAACTCAG
AAATATATGCTCACTCCCCATGGCATGTTTGAGTTGAAGTATTTTTTTGGCAGCCACGTCGCAACCGAAGCGGGTGGGGA
AGCTTCCTCGACTGCGATCCGTGCGCTGATCGTGCAATTCACAGGAGCCGAAGACCCGAAGAACCCTTTATCCGACAGTA
AGATTGCGGACATGCTGGGAGAACAGGGCATGGTGATTGCGCGACGCACTGTTGCCAAGTATCGCGAAGCCTTGAAAATC
CCTCCCGTCAGCCTCCGCAAGTCCTTGTAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(333-487)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.99

100

0.63

Multiple sequence alignment