Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AHTJR_RS01425 Genome accession   NZ_CP038009
Coordinates   308292..309029 (+) Length   245 a.a.
NCBI ID   WP_134251340.1    Uniprot ID   A0A4P7B1S1
Organism   Acinetobacter haemolyticus strain TJR01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303292..314029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AHTJR_RS01410 (AHTJR_01410) ponA 303839..306430 (-) 2592 WP_134251338.1 penicillin-binding protein PBP1a -
  AHTJR_RS01415 (AHTJR_01415) comM 306594..307652 (+) 1059 WP_004641327.1 pilus assembly protein PilM Machinery gene
  AHTJR_RS01420 (AHTJR_01420) pilN 307652..308362 (+) 711 WP_410169532.1 PilN domain-containing protein Machinery gene
  AHTJR_RS01425 (AHTJR_01425) pilO 308292..309029 (+) 738 WP_134251340.1 type 4a pilus biogenesis protein PilO Machinery gene
  AHTJR_RS01430 (AHTJR_01430) pilP 309041..309568 (+) 528 WP_134251342.1 pilus assembly protein PilP Machinery gene
  AHTJR_RS01435 (AHTJR_01435) pilQ 309630..311762 (+) 2133 WP_410169539.1 type IV pilus secretin PilQ Machinery gene
  AHTJR_RS01440 (AHTJR_01440) aroK 311801..312343 (+) 543 WP_005087029.1 shikimate kinase AroK -
  AHTJR_RS01445 (AHTJR_01445) aroB 312363..313448 (+) 1086 WP_004641316.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 28052.08 Da        Isoelectric Point: 9.1421

>NTDB_id=351226 AHTJR_RS01425 WP_134251340.1 308292..309029(+) (pilO) [Acinetobacter haemolyticus strain TJR01]
MRPKEMNDLNQEGAAVKKKKMTVEQFFQQFNTLDMNNYGSWPWSVKVTCWIFILLAVAALGYFVVIQKQLQTIANAEARE
QSLLNEFREKESKLRNLQQYQTQLQEMEANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEKEVRQEFFIEQP
ISLEATGDYHAFGAFATGIAALPRIVTLHDFIVEAKEDTQKKSDIPVVHYTVKAKTYRYVGADDRAVGNTSAPIASKKNK
QSKKK

Nucleotide


Download         Length: 738 bp        

>NTDB_id=351226 AHTJR_RS01425 WP_134251340.1 308292..309029(+) (pilO) [Acinetobacter haemolyticus strain TJR01]
ATGAGACCAAAAGAAATGAACGATCTCAATCAAGAAGGCGCAGCTGTAAAGAAAAAGAAAATGACAGTTGAGCAGTTCTT
TCAGCAATTTAATACCTTGGATATGAATAACTATGGCAGTTGGCCATGGTCGGTAAAAGTGACGTGTTGGATTTTTATTT
TACTCGCTGTTGCGGCATTAGGTTATTTTGTTGTGATCCAAAAGCAGCTACAGACGATAGCTAATGCTGAAGCGCGTGAA
CAAAGTCTATTAAATGAGTTTAGAGAAAAGGAATCTAAATTACGCAACTTGCAGCAATACCAAACCCAGTTGCAAGAAAT
GGAAGCGAATTTTAATCAACAGCTTGAGCAGTTGCCAAAAGAAACTGAAATTCCGAGTTTGGTTGAGGATATAAACTTAA
CTGGGGTAAATTCAGGTCTGAAATTTAAAAATATTCGTTTAGAAAAAGAAGTTAGACAAGAGTTCTTCATTGAGCAGCCA
ATTAGTCTGGAAGCAACAGGCGATTATCATGCCTTCGGTGCTTTTGCGACAGGTATCGCCGCTTTGCCTCGAATCGTGAC
TTTGCATGATTTTATCGTCGAAGCGAAAGAAGATACTCAGAAGAAATCTGATATCCCTGTTGTCCATTATACGGTTAAAG
CAAAAACTTATCGTTATGTTGGGGCGGATGATCGGGCTGTAGGTAACACTTCGGCTCCAATCGCATCTAAAAAAAATAAA
CAGTCGAAGAAGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7B1S1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

72.034

96.327

0.694
  comO Acinetobacter baylyi ADP1

71.121

94.694

0.673

Multiple sequence alignment