Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KKOR_RS03550 Genome accession   NC_013166
Coordinates   719444..721156 (+) Length   570 a.a.
NCBI ID   WP_012800640.1    Uniprot ID   C7R9N5
Organism   Kangiella koreensis DSM 16069     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714444..726156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKOR_RS03525 (Kkor_0701) ampD 714702..715247 (+) 546 WP_041296120.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KKOR_RS03530 (Kkor_0702) nadC 715371..716246 (+) 876 WP_012800636.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KKOR_RS03535 (Kkor_0703) - 716373..716678 (+) 306 WP_012800637.1 GIY-YIG nuclease family protein -
  KKOR_RS13710 (Kkor_0704) - 717125..717571 (+) 447 WP_012800638.1 pilin -
  KKOR_RS03545 (Kkor_0705) - 717642..719351 (+) 1710 WP_012800639.1 PglL family O-oligosaccharyltransferase -
  KKOR_RS03550 (Kkor_0706) pilB 719444..721156 (+) 1713 WP_012800640.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KKOR_RS03555 (Kkor_0707) pilC 721280..722524 (+) 1245 WP_012800641.1 type II secretion system F family protein Machinery gene
  KKOR_RS03560 (Kkor_0708) pilD 722567..723475 (+) 909 WP_012800642.1 A24 family peptidase Machinery gene
  KKOR_RS03565 (Kkor_0709) coaE 723528..724127 (+) 600 WP_012800643.1 dephospho-CoA kinase -
  KKOR_RS03570 (Kkor_0710) zapD 724135..724902 (+) 768 WP_012800644.1 cell division protein ZapD -
  KKOR_RS03575 (Kkor_0711) ampE 725006..725875 (+) 870 WP_012800645.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62475.91 Da        Isoelectric Point: 5.2676

>NTDB_id=35079 KKOR_RS03550 WP_012800640.1 719444..721156(+) (pilB) [Kangiella koreensis DSM 16069]
MTLADTGLSGLAHLLVAEGLLTKEKVQQAVKNAKEEKVGLIDWLVAAGWLEGKQAAKAQALNFGYPYFDLSSIAFENIPK
DVIDLKLMQAHRAVPLYKRGTRLFVAVSDATNLKSLEEIRFQTGCSVEAVLVEPNKLESVVGKLIEEKENETLSDFDDSD
LDNIDLDAVDPEAEQEMDDKAAGEDAPVVKFVKQMLISAIRKGASDLHFEPYEKKYRVRFRIDGILHEIASPPIQLASRI
SARLKVLSGLDISERRVPQDGRVKLKISKTKAIDFRVNTLPTLFGEKIVMRILDPAQAMLGIDALGFDPKQKKDFMGAIE
KPQGMVLVTGPTGSGKTVTLYTGVNILNDEYKNISTAEDPVEINLHGVNQVNVNVKAGLTFASALRAFLRQDPDIVLVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLVNMGVAPFNIATTVNLVVAQRLTRRLCEHCKKPSDLPKEALIEE
GFSEEELNELTIYEPVGCDKCTMGYKGRVGLFQVMPVSEESGRIIMEGGNAINIADQAKKEGIPDLRRAGLNKVKAGLTS
LAEVNRVTQE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=35079 KKOR_RS03550 WP_012800640.1 719444..721156(+) (pilB) [Kangiella koreensis DSM 16069]
ATGACTTTAGCGGATACAGGCCTTTCCGGATTAGCTCATTTATTGGTAGCTGAAGGACTTTTAACGAAAGAAAAGGTCCA
GCAGGCAGTCAAAAATGCCAAGGAAGAAAAAGTTGGTTTAATTGACTGGCTGGTAGCAGCAGGCTGGTTGGAGGGGAAGC
AGGCAGCTAAAGCTCAAGCTCTCAATTTTGGTTATCCTTATTTCGACCTCTCCAGTATAGCCTTCGAAAATATTCCTAAA
GATGTCATTGATCTTAAGCTTATGCAGGCACACAGGGCTGTTCCTCTCTACAAGCGAGGCACTCGTCTATTTGTCGCAGT
ATCTGACGCGACTAACCTGAAAAGTTTAGAAGAAATTCGATTCCAAACAGGCTGCTCGGTTGAAGCAGTCTTGGTGGAGC
CGAATAAGCTTGAGTCGGTAGTCGGTAAGCTGATTGAAGAAAAAGAAAATGAAACCCTGTCTGATTTCGATGATTCCGAC
CTGGATAATATTGATTTAGATGCTGTAGACCCTGAAGCTGAGCAGGAAATGGATGATAAAGCTGCCGGTGAAGATGCGCC
AGTAGTTAAGTTTGTTAAGCAAATGCTCATTTCAGCGATTCGTAAAGGTGCTTCCGATTTACACTTTGAGCCTTATGAAA
AAAAATATCGTGTTCGTTTTCGGATTGATGGTATTTTGCATGAAATTGCATCACCACCTATACAGCTTGCCAGCCGTATA
TCTGCACGCTTAAAAGTTCTTTCTGGGCTGGATATTTCAGAACGTCGTGTTCCTCAAGATGGTCGTGTAAAACTAAAAAT
TTCAAAAACAAAAGCGATTGATTTTCGTGTTAATACGCTGCCTACTTTGTTTGGTGAGAAAATTGTAATGCGTATTCTAG
ATCCCGCGCAGGCAATGTTAGGTATTGACGCTTTAGGATTTGACCCAAAGCAGAAAAAAGATTTCATGGGTGCTATTGAA
AAGCCTCAAGGAATGGTGTTGGTAACAGGCCCTACTGGCTCTGGTAAAACAGTAACTCTCTATACTGGTGTGAATATTCT
TAACGATGAATATAAAAATATTTCTACCGCAGAAGACCCTGTAGAGATTAATTTACATGGTGTTAACCAGGTCAATGTTA
ATGTCAAAGCAGGTTTAACCTTTGCTTCGGCACTGAGGGCTTTTTTACGTCAGGATCCAGATATTGTGCTGGTGGGTGAG
ATTCGAGACCTTGAAACCGCTGAAATTGCCATTAAAGCCGCCCAGACCGGTCACTTGGTATTATCTACCCTGCATACTAA
TTCAGCACCAGAAACTTTGACTCGGTTAGTTAATATGGGGGTTGCACCATTTAATATTGCTACCACGGTAAACTTGGTGG
TAGCTCAGCGTCTGACAAGGCGTCTCTGCGAACACTGCAAGAAACCATCAGATTTACCTAAAGAAGCATTGATTGAAGAG
GGTTTTAGCGAAGAAGAGCTGAACGAACTCACAATTTATGAGCCCGTAGGTTGCGATAAATGCACAATGGGCTATAAAGG
TCGTGTGGGCTTGTTCCAAGTTATGCCAGTTTCGGAAGAAAGTGGTCGCATTATTATGGAAGGAGGTAATGCAATCAATA
TTGCTGATCAAGCTAAAAAAGAGGGGATTCCAGACTTACGTAGAGCAGGCCTTAATAAGGTCAAAGCGGGACTGACCAGT
TTGGCGGAAGTCAACCGTGTAACTCAAGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-146)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C7R9N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.657

99.298

0.582
  pilB Acinetobacter baumannii D1279779

58.23

99.123

0.577
  pilB Legionella pneumophila strain ERS1305867

55.752

99.123

0.553
  pilF Neisseria gonorrhoeae MS11

49.559

99.474

0.493
  pilB Vibrio cholerae strain A1552

47.803

99.825

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

44.852

100

0.451
  pilB Vibrio campbellii strain DS40M4

45.179

98.246

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.437

99.649

0.393
  pilF Thermus thermophilus HB27

39.539

98.947

0.391

Multiple sequence alignment