Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EZ304_RS07065 Genome accession   NZ_CP037858
Coordinates   1547033..1548091 (-) Length   352 a.a.
NCBI ID   WP_099552984.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain X28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1542033..1553091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZ304_RS07045 - 1542971..1544938 (-) 1968 WP_111205121.1 type IV pilus secretin PilQ family protein -
  EZ304_RS07050 - 1544960..1545493 (-) 534 WP_227843533.1 pilus assembly protein PilP -
  EZ304_RS07055 pilO 1545490..1546146 (-) 657 WP_005410857.1 type 4a pilus biogenesis protein PilO -
  EZ304_RS07060 - 1546143..1547033 (-) 891 WP_142806654.1 PilN domain-containing protein -
  EZ304_RS07065 pilM 1547033..1548091 (-) 1059 WP_099552984.1 pilus assembly protein PilM Machinery gene
  EZ304_RS07070 - 1548311..1550734 (+) 2424 WP_142806655.1 penicillin-binding protein 1A -
  EZ304_RS07075 - 1551042..1551686 (+) 645 WP_142806656.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37713.30 Da        Isoelectric Point: 4.4047

>NTDB_id=350234 EZ304_RS07065 WP_099552984.1 1547033..1548091(-) (pilM) [Stenotrophomonas maltophilia strain X28]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKLAAAA
VAGSAVITKVIPMPAELDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGAIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSSDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=350234 EZ304_RS07065 WP_099552984.1 1547033..1548091(-) (pilM) [Stenotrophomonas maltophilia strain X28]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTTGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGCGGCAATCGTTTTCGTGTGGAACATTACGCTGTGGAACCTCTTCCGCCGAATGCGGTGGTAGAGAAGAACATCG
TGGAAGTGGAGGCCGTGGGTGAGGCCATCCGCCGTGCGATGAACCGTTCAGGCAGCAAGGCCAAGCTGGCCGCTGCCGCC
GTCGCCGGGTCCGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGAGCTCGACGAGAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAATTACATTCCGTACCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGGCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAACGTGGAACTGCGCCAGTCCGCGCTGGAACTGGGT
GGGCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTACGCCCTGGTCGCCAGTGAGCTGCCGGT
GTCCAGCGATGGCGTGGTCGCGCTGGTCGACATCGGCGCCACCATGACCACCCTCAATGTCCTGCGCGGTGGCCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGTGGCTTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTATGCGGGCAGTGAGTTCAATCGCGTCGACCACATCGTGCTGGCCGGCG
GTTGCGCCGTACTGGGTGGCCTGCCGGAGATGGTCGAAGAACAGCTGGGCGTGCCGACCGTGGTCGCCAATCCGCTGGCA
CAGATGACCCTGGGCCCGAAGGTGAACGCGCATGCGCTGGCCCAGGATGCCCCCGCGCTGATGATCGCCACCGGTCTGGC
GCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.136

100

0.511
  comM Acinetobacter nosocomialis M2

51.136

100

0.511
  comM Acinetobacter baylyi ADP1

50

100

0.5
  pilM Legionella pneumophila strain ERS1305867

47.863

99.716

0.477

Multiple sequence alignment