Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   E0Z08_RS16975 Genome accession   NZ_CP037424
Coordinates   3414239..3414976 (-) Length   245 a.a.
NCBI ID   WP_005401353.1    Uniprot ID   A0A7H8SJL8
Organism   Acinetobacter johnsonii strain M19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3409239..3419976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0Z08_RS19275 (E0Z08_16950) - 3410036..3410518 (-) 483 WP_265937476.1 shikimate kinase -
  E0Z08_RS16955 (E0Z08_16955) - 3410607..3411884 (-) 1278 Protein_3284 type IV pilus secretin PilQ -
  E0Z08_RS16960 (E0Z08_16960) - 3411941..3412890 (+) 950 Protein_3285 IS481 family transposase -
  E0Z08_RS16965 (E0Z08_16965) - 3412901..3413647 (-) 747 Protein_3286 AMIN domain-containing protein -
  E0Z08_RS16970 (E0Z08_16970) pilP 3413712..3414239 (-) 528 WP_005401352.1 pilus assembly protein PilP Machinery gene
  E0Z08_RS16975 (E0Z08_16975) pilO 3414239..3414976 (-) 738 WP_005401353.1 type 4a pilus biogenesis protein PilO Machinery gene
  E0Z08_RS16980 (E0Z08_16980) pilN 3414973..3415629 (-) 657 WP_005401354.1 PilN domain-containing protein Machinery gene
  E0Z08_RS16985 (E0Z08_16985) - 3415629..3416687 (-) 1059 Protein_3290 pilus assembly protein PilM -
  E0Z08_RS16990 (E0Z08_16990) ponA 3416854..3419382 (+) 2529 WP_005401356.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27705.26 Da        Isoelectric Point: 5.1834

>NTDB_id=349705 E0Z08_RS16975 WP_005401353.1 3414239..3414976(-) (pilO) [Acinetobacter johnsonii strain M19]
MSRDEFEELTSENHAVAKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQVQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNDKKTDIPQISYSVKAKTYRYVGATATTDVDVKDTKNDKKAD
AGGAK

Nucleotide


Download         Length: 738 bp        

>NTDB_id=349705 E0Z08_RS16975 WP_005401353.1 3414239..3414976(-) (pilO) [Acinetobacter johnsonii strain M19]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAGCCAAGAAAAAAATGACGGTTGATAAATTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGTAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAATTTGCAGCAATATCAAGTGCAATTGCAAGAAAT
GGAAGCCAATTTTAATCAGCAGTTAGAACAGCTGCCGAAAGAAACTGAAATTCCTGGCTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAACCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTTAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAACAATGATAAAAAGACGGATATTCCGCAAATTTCATATAGTGTAAAAG
CGAAAACCTACCGTTATGTCGGGGCAACGGCTACAACGGATGTTGATGTAAAAGATACAAAAAATGATAAGAAAGCCGAT
GCGGGAGGTGCCAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8SJL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

74.89

92.653

0.694
  comO Acinetobacter baylyi ADP1

72.398

90.204

0.653

Multiple sequence alignment