Detailed information NTG0348239

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CEA76_RS02810
• Genome accession: NZ_CP036371
• Coordinates: 554461..555261 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain WCHKP020037
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 549461..560261

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CEA76_RS02790 (CEA76_02790)	dksA	550041..550496 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
CEA76_RS02795 (CEA76_02795)	sfsA	550659..551375 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
CEA76_RS02800 (CEA76_02800)	thpR	551375..551911 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
CEA76_RS02805 (CEA76_02805)	hrpB	551984..554413 (+)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
CEA76_RS02810 (CEA76_02810)	pilD	554461..555261 (-)	801	WP_002888947.1	prepilin peptidase	Machinery gene
CEA76_RS02815 (CEA76_02815)	-	555258..556004 (-)	747	WP_004145897.1	type II secretion system protein N	-
CEA76_RS02820 (CEA76_02820)	-	555994..556479 (-)	486	WP_004151943.1	type II secretion system protein M	-
CEA76_RS02825 (CEA76_02825)	gspL	556472..557665 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
CEA76_RS02830 (CEA76_02830)	gspK	557652..558638 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
CEA76_RS02835 (CEA76_02835)	gspJ	558635..559231 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
CEA76_RS02840 (CEA76_02840)	gspI	559228..559593 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
CEA76_RS02845 (CEA76_02845)	gspH	559590..560105 (-)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=348239 CEA76_RS02810 WP_002888947.1 554461..555261(-) (pilD) [Klebsiella pneumoniae strain WCHKP020037]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=348239 CEA76_RS02810 WP_002888947.1 554461..555261(-) (pilD) [Klebsiella pneumoniae strain WCHKP020037]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425