Detailed information NTG0345159

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C9E88_RS07845
• Genome accession: NZ_CP035934
• Coordinates: 1638839..1640026 (+)
• Length: 395 a.a.
• NCBI ID: WP_106985200.1
• Uniprot ID: -
• Organism: Acinetobacter cumulans strain WCHAc060092
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1633839..1645026

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C9E88_RS07830 (C9E88_012605)	-	1634707..1635723 (+)	1017	WP_106985203.1	FAD-binding oxidoreductase	-
C9E88_RS07835 (C9E88_012600)	-	1635746..1636900 (+)	1155	WP_106985202.1	acyl-CoA desaturase	-
C9E88_RS07840 (C9E88_012595)	-	1637512..1638567 (+)	1056	WP_106985201.1	3-deoxy-7-phosphoheptulonate synthase	-
C9E88_RS07845 (C9E88_012590)	pilU	1638839..1640026 (+)	1188	WP_106985200.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C9E88_RS07850 (C9E88_012585)	gcvH	1640264..1640638 (-)	375	WP_106985199.1	glycine cleavage system protein GcvH	-
C9E88_RS07855 (C9E88_012580)	-	1640723..1641751 (-)	1029	WP_106985198.1	AraC family transcriptional regulator	-
C9E88_RS07860 (C9E88_012575)	-	1641901..1642344 (-)	444	WP_106985197.1	MaoC family dehydratase	-
C9E88_RS07865 (C9E88_012570)	-	1642387..1644216 (-)	1830	WP_106985196.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 395 a.a.
• Molecular weight: 44531.94 Da
• Isoelectric Point: 6.3933

>NTDB_id=345159 C9E88_RS07845 WP_106985200.1 1638839..1640026(+) (pilU) [Acinetobacter cumulans strain WCHAc060092]
MYSAELLEEARRLMFHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQALTPDKTKLFAYSLMNEKQRKEFDAELEC
NFAISVPEISRFRVNVFQQQLNVGMVIRTITNEIPTFTQLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTEDGKGRRAAVEIMLNTPLISDLILKGEFHELKGIMARS
RELGMQTFDQALFDLYNEGAISYDEALRNADSTNELRLQIKLKSSRANPALESTSSFSMMSESKEEEPAEGEKAS

Nucleotide

• Length: 1188 bp

>NTDB_id=345159 C9E88_RS07845 WP_106985200.1 1638839..1640026(+) (pilU) [Acinetobacter cumulans strain WCHAc060092]
ATGTACTCAGCTGAGTTGCTTGAAGAAGCACGTCGCTTAATGTTCCACATGTTAACCAAAGTTGTTGAGTACGGCGGTTC
TGACTTATTCATTACGGCAGATTTTCCACCGAGTATTAAGCACCAAGGTCTAATGAAGCCTTTTGGTCAACAAGCATTAA
CACCAGATAAAACCAAATTATTTGCATATAGCTTAATGAATGAAAAGCAACGTAAAGAGTTTGATGCGGAACTAGAATGT
AACTTTGCGATTAGTGTGCCAGAAATTTCACGTTTTCGTGTGAATGTATTTCAGCAACAATTAAATGTAGGTATGGTCAT
TCGTACCATTACCAATGAAATTCCAACATTTACCCAGTTAAAATTGCCCGAATCACTTAAGCATGTGATTATGGAAAAAC
GTGGTTTGGTATTGGTTGTTGGTGGTACAGGCTCTGGTAAGTCAACTTCCTTGGCTGCCATGATTGACCATCGCAATGAA
AATTCAGCTGGTCATATTATTACGGTTGAAGACCCAGTCGAATATGTACATAAGCATAAAAAATCGATGATTACTCACCG
TGAAGTTGGCGTGGATTGTCATTCTTGGCACAACGCGTTAAAAAATACCTTGCGCCAAGCTCCTGATGTTATTTTAATCG
GTGAGATCCGTGATACTGAAACCATGGAACATGCGATTGCTTTTGCTGAAACAGGGCACTTATGTTTGGGGACTTTACAT
GCTAATAATGCCAACCAAACCTTGGACCGAATTATTAACTTCTTCCCAGAAGAGCGTAGAAATCAGTTGCTTATGGACTT
GTCGTCTAATATGAAGGCGATTATTTCGCAACGTTTAATTCGTACAGAAGATGGTAAAGGGCGCCGTGCTGCTGTTGAAA
TTATGTTGAATACACCGTTAATTTCAGACTTGATTTTAAAAGGTGAATTCCATGAATTAAAAGGAATTATGGCAAGATCA
CGTGAGCTTGGTATGCAGACTTTTGATCAGGCATTATTTGATTTATATAATGAAGGCGCAATTTCGTATGATGAAGCGCT
TCGTAATGCCGATTCAACCAATGAGTTACGTTTACAAATTAAATTGAAAAGTAGCCGTGCAAACCCTGCACTCGAGTCAA
CCTCTAGTTTTAGTATGATGAGTGAAAGCAAGGAAGAAGAGCCCGCTGAAGGTGAGAAGGCATCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (102-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.981	94.43	0.557
pilU	Acinetobacter baylyi ADP1	56.712	92.405	0.524
pilU	Vibrio cholerae strain A1552	53.179	87.595	0.466
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.323	94.177	0.38