Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NT05HA_RS06720 Genome accession   NC_012913
Coordinates   1469889..1470338 (+) Length   149 a.a.
NCBI ID   WP_005701262.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus NJ8700     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1464889..1475338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NT05HA_RS06705 (NT05HA_1460) pnp 1465869..1467998 (-) 2130 WP_005701259.1 polyribonucleotide nucleotidyltransferase -
  NT05HA_RS06710 (NT05HA_1461) nanQ 1468268..1468738 (+) 471 WP_005701260.1 N-acetylneuraminate anomerase -
  NT05HA_RS06715 (NT05HA_1462) ampD 1469210..1469764 (-) 555 WP_012771713.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NT05HA_RS06720 (NT05HA_1464) pilA 1469889..1470338 (+) 450 WP_005701262.1 pilin Machinery gene
  NT05HA_RS06725 (NT05HA_1465) pilB 1470363..1471772 (+) 1410 WP_005701263.1 GspE/PulE family protein Machinery gene
  NT05HA_RS06730 (NT05HA_1466) pilC 1471765..1473003 (+) 1239 WP_005701265.1 type II secretion system F family protein Machinery gene
  NT05HA_RS06735 (NT05HA_1467) - 1473000..1473692 (+) 693 WP_005701266.1 prepilin peptidase -
  NT05HA_RS06740 (NT05HA_1468) coaE 1473745..1474365 (+) 621 WP_005701267.1 dephospho-CoA kinase -
  NT05HA_RS06745 (NT05HA_1469) yacG 1474358..1474570 (+) 213 WP_005701268.1 DNA gyrase inhibitor YacG -
  NT05HA_RS06750 (NT05HA_1470) - 1474570..1474836 (+) 267 WP_005701270.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15303.75 Da        Isoelectric Point: 8.8087

>NTDB_id=34470 NT05HA_RS06720 WP_005701262.1 1469889..1470338(+) (pilA) [Aggregatibacter aphrophilus NJ8700]
MKIQSAFLSVKSVKKGFTLIELMIVIAIVAILATVAIPSYQNYTKKAAVSELLQASAPLRSEVELCIYNTGSVENCTGGK
NGIQADVADATAKYVKSTAVKGGIITVVGKGSLDKVSYTLTAAGSATAGVTWTASCGTNADIFPAGFCS

Nucleotide


Download         Length: 450 bp        

>NTDB_id=34470 NT05HA_RS06720 WP_005701262.1 1469889..1470338(+) (pilA) [Aggregatibacter aphrophilus NJ8700]
ATGAAAATTCAGTCAGCATTTCTTTCTGTGAAATCCGTTAAAAAAGGATTTACATTAATTGAATTAATGATCGTGATTGC
GATTGTCGCCATTTTGGCTACTGTCGCGATTCCATCTTACCAAAATTACACCAAAAAAGCGGCAGTTTCCGAATTGTTAC
AAGCCTCTGCTCCACTTCGTTCCGAAGTCGAACTGTGCATTTACAATACGGGTAGTGTTGAGAACTGCACCGGCGGAAAA
AATGGCATTCAAGCCGATGTCGCCGACGCTACCGCAAAATATGTGAAATCAACCGCAGTGAAAGGCGGCATCATTACCGT
GGTCGGAAAAGGCAGCTTGGATAAAGTGTCTTACACACTAACCGCGGCAGGTTCTGCAACTGCCGGCGTCACATGGACAG
CCTCTTGCGGCACTAACGCCGACATCTTTCCGGCCGGCTTCTGTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

65.468

93.289

0.611
  pilA Haemophilus influenzae Rd KW20

63.83

94.631

0.604
  pilA Glaesserella parasuis strain SC1401

55.245

95.973

0.53

Multiple sequence alignment