Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   EWS90_RS25895 Genome accession   NZ_CP035739
Coordinates   5359569..5360906 (-) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa strain 1334/14     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5354569..5365906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWS90_RS25865 (EWS90_25865) pilX 5355220..5355807 (-) 588 WP_034037187.1 type 4a pilus minor pilin PilX -
  EWS90_RS25870 (EWS90_25870) pilW 5355804..5356628 (-) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  EWS90_RS25875 (EWS90_25875) pilV 5356625..5357182 (-) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  EWS90_RS25880 (EWS90_25880) - 5357173..5357679 (-) 507 WP_003102598.1 Tfp pilus assembly protein FimT/FimU -
  EWS90_RS25885 (EWS90_25885) - 5357785..5358294 (-) 510 WP_003161431.1 Tfp pilus assembly protein FimT/FimU -
  EWS90_RS25890 (EWS90_25890) thiO 5358429..5359523 (+) 1095 WP_023084775.1 glycine oxidase ThiO -
  EWS90_RS25895 (EWS90_25895) pilR 5359569..5360906 (-) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  EWS90_RS25900 (EWS90_25900) pilS 5360921..5362513 (-) 1593 WP_003123394.1 two-component system sensor histidine kinase PilS Regulator
  EWS90_RS25905 (EWS90_25905) - 5362503..5362748 (-) 246 WP_003102591.1 PP0621 family protein -
  EWS90_RS25910 (EWS90_25910) - 5362905..5363930 (-) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  EWS90_RS25915 (EWS90_25915) rluD 5364076..5365038 (+) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EWS90_RS25920 (EWS90_25920) pgeF 5365035..5365763 (+) 729 WP_003112833.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=344193 EWS90_RS25895 WP_003094694.1 5359569..5360906(-) (pilR) [Pseudomonas aeruginosa strain 1334/14]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=344193 EWS90_RS25895 WP_003094694.1 5359569..5360906(-) (pilR) [Pseudomonas aeruginosa strain 1334/14]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTAGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

100

100

1
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment