Detailed information NTG0342773

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EUZ85_RS10380
• Genome accession: NZ_CP035490
• Coordinates: 2307500..2308714 (-)
• Length: 404 a.a.
• NCBI ID: WP_127969228.1
• Uniprot ID: A0AAE5XHU2
• Organism: Hahella sp. KA22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2302500..2313714

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EUZ85_RS10355 (EUZ85_10355)	-	2302683..2303345 (-)	663	WP_127969223.1	SDR family oxidoreductase	-
EUZ85_RS10360 (EUZ85_10360)	-	2303429..2303998 (-)	570	WP_127969224.1	NADPH-dependent FMN reductase	-
EUZ85_RS10365 (EUZ85_10365)	-	2304365..2304961 (+)	597	WP_127969225.1	DUF938 domain-containing protein	-
EUZ85_RS10370 (EUZ85_10370)	-	2305013..2305666 (-)	654	WP_127969226.1	DUF4291 domain-containing protein	-
EUZ85_RS10375 (EUZ85_10375)	-	2305934..2307310 (-)	1377	WP_127969227.1	wax ester/triacylglycerol synthase family O-acyltransferase	-
EUZ85_RS10380 (EUZ85_10380)	pilU	2307500..2308714 (-)	1215	WP_127969228.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EUZ85_RS10385 (EUZ85_10385)	-	2309003..2311549 (+)	2547	WP_127969229.1	EAL domain-containing protein	-
EUZ85_RS10390 (EUZ85_10390)	-	2311603..2312427 (-)	825	WP_127969230.1	OmpA family protein	-
EUZ85_RS10395 (EUZ85_10395)	-	2312570..2313388 (-)	819	WP_127969231.1	alpha/beta fold hydrolase	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 44867.14 Da
• Isoelectric Point: 5.4889

>NTDB_id=342773 EUZ85_RS10380 WP_127969228.1 2307500..2308714(-) (pilU) [Hahella sp. KA22]
MEFNQYLTVLAKNDGSDLYLSTGAPPCAKFHGKLKPIDKTPIPPGRIMEIANSIMDEEQREQFAHELEMNLAYSIPQVGR
FRVNIFKQRNEVSIVARNIVTDIPNADSLGLPPILKEVLMSKRGLILFVGATGSGKSTSLAALIDHRNSNSGGHIITIED
PVEFIHKHKKSIINQREVGVDTRSYHNALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLCISTLHANNSNQALDRI
INFFPEERRNQLLMDLSLNLRAFVSQRLVPTIDGKRTAAIEILLGTPTVSELILRGDIDGIKEIMQKSENIGMQTFDAAL
FKLVQQEKISVEEALKNADSANNLRLRIKLHAGVEVPTSLGDEKVEDAGPEELAAKKPSSGFSLSLQEIEEDDEEDNDPF
KPKS

Nucleotide

• Length: 1215 bp

>NTDB_id=342773 EUZ85_RS10380 WP_127969228.1 2307500..2308714(-) (pilU) [Hahella sp. KA22]
ATGGAATTCAACCAGTATCTGACCGTTCTGGCCAAGAATGACGGGTCCGACCTCTACCTGAGCACCGGCGCGCCGCCCTG
CGCCAAGTTTCACGGTAAATTGAAGCCCATCGATAAGACCCCCATTCCGCCGGGCAGAATTATGGAAATCGCCAACTCCA
TCATGGATGAAGAACAGCGCGAGCAGTTCGCCCATGAGCTGGAAATGAATCTGGCGTACAGCATCCCTCAGGTTGGCCGT
TTCCGGGTCAACATCTTCAAACAGCGCAATGAAGTCTCCATCGTCGCCCGTAATATCGTGACCGATATTCCCAACGCTGA
CAGCCTGGGGCTGCCCCCTATCCTGAAAGAAGTATTGATGTCGAAACGGGGCTTGATTCTATTCGTCGGCGCTACCGGCT
CCGGCAAGTCCACCTCTCTGGCGGCGCTGATCGATCACCGCAACAGTAATAGCGGCGGTCATATCATTACTATCGAAGAC
CCGGTGGAGTTCATTCACAAGCACAAGAAAAGCATCATCAACCAGCGGGAAGTGGGCGTCGATACGCGCAGTTACCATAA
CGCGCTGAAAAACACCCTGCGGCAGGCGCCGGACGTCATTTTGATCGGCGAGATTCGCGACAGGGAAACCATGGAGCACG
CGCTGGCTTTCGCAGAAACCGGGCACCTGTGTATCTCCACGCTGCACGCCAACAACTCTAACCAGGCGCTGGACCGCATC
ATTAACTTTTTCCCGGAAGAACGCCGCAACCAGTTGCTGATGGATTTGTCGTTGAACCTGCGAGCCTTCGTCTCACAACG
TTTGGTGCCGACGATTGACGGTAAGCGCACCGCCGCCATCGAGATCCTGCTGGGTACGCCCACGGTCAGCGAATTGATTC
TGCGCGGCGACATCGACGGCATCAAAGAGATCATGCAGAAGTCGGAAAACATCGGCATGCAGACCTTTGACGCAGCGCTG
TTCAAGCTGGTGCAGCAGGAAAAAATCAGCGTGGAGGAAGCCCTGAAAAACGCCGACTCCGCCAACAACCTGCGTCTGCG
CATCAAACTGCACGCCGGGGTGGAAGTTCCCACCAGCCTGGGCGATGAAAAAGTTGAGGACGCCGGCCCCGAAGAACTGG
CGGCCAAGAAGCCCAGCAGCGGTTTTTCTCTCAGCCTGCAGGAAATTGAAGAGGACGACGAGGAGGACAACGACCCCTTC
AAACCTAAAAGTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.835	86.881	0.502
pilU	Acinetobacter baylyi ADP1	56.534	87.129	0.493
pilU	Vibrio cholerae strain A1552	49.435	87.624	0.433
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.576	94.554	0.384