Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EUZ85_RS09235 Genome accession   NZ_CP035490
Coordinates   2062873..2064585 (-) Length   570 a.a.
NCBI ID   WP_127969022.1    Uniprot ID   A0AAE5XGW4
Organism   Hahella sp. KA22     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057873..2069585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUZ85_RS09205 (EUZ85_09205) tsaA 2058015..2058731 (-) 717 WP_127969016.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  EUZ85_RS09210 (EUZ85_09210) - 2058741..2059610 (-) 870 WP_127969017.1 hypothetical protein -
  EUZ85_RS09215 (EUZ85_09215) yacG 2059832..2060068 (-) 237 WP_127969018.1 DNA gyrase inhibitor YacG -
  EUZ85_RS09220 (EUZ85_09220) coaE 2060058..2060684 (-) 627 WP_127969019.1 dephospho-CoA kinase -
  EUZ85_RS09225 (EUZ85_09225) pilD 2060704..2061582 (-) 879 WP_127969020.1 A24 family peptidase Machinery gene
  EUZ85_RS09230 (EUZ85_09230) pilC 2061649..2062869 (-) 1221 WP_127969021.1 type II secretion system F family protein Machinery gene
  EUZ85_RS09235 (EUZ85_09235) pilB 2062873..2064585 (-) 1713 WP_127969022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EUZ85_RS09240 (EUZ85_09240) pilE 2064906..2065349 (+) 444 WP_127969023.1 pilin Machinery gene
  EUZ85_RS09245 (EUZ85_09245) - 2065529..2067325 (+) 1797 WP_127969024.1 hypothetical protein -
  EUZ85_RS09250 (EUZ85_09250) - 2067358..2068422 (+) 1065 WP_127969025.1 UDP-N-acetylglucosamine 2-epimerase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62795.79 Da        Isoelectric Point: 5.3666

>NTDB_id=342769 EUZ85_RS09235 WP_127969022.1 2062873..2064585(-) (pilB) [Hahella sp. KA22]
MNTQISPPLTGLARRFVQDGLLEESVAREAQANAQKSKDPFVSYIVKQGLANATQLAYAASAEFGVPLMDLSGLSIEQIP
DKIVDEKLIRKHHALPIFKRGNRLFLAVADPTNLQALDEIKFHTGLGTDAILVEEDKLSALIEKYLDSHEQPLGDLGDTE
LDDIDIDAGDEPDRADDSGPEQDDAPIVKYINKMLLDAIKGGASDIHFEPYEKSYRVRFRTDGILNEVSKPSIKLAAKLA
ARLKVMSQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEEDQKKMYLDALAQ
PQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGVNQVNVNTRVGLDFSSALRAFLRQDPDVVMVGEI
RDLDTASIAIKAAQTGHLVLSTLHTNSAAETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCSECKETITVPNDVLKEEG
FTDKQIETGFKLYRPKGCDKCKDGYKGRVGIYEVVRITPAMQELIMNEASSLELAKQAQKEGFRNLRQSALRKVIEGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=342769 EUZ85_RS09235 WP_127969022.1 2062873..2064585(-) (pilB) [Hahella sp. KA22]
ATGAATACACAGATTTCCCCGCCATTAACAGGGTTAGCGCGTCGCTTCGTTCAGGATGGGCTGCTAGAAGAGAGCGTAGC
ACGTGAGGCGCAAGCCAACGCGCAAAAGTCCAAGGACCCTTTTGTTAGCTATATCGTCAAGCAAGGTCTAGCCAACGCCA
CACAACTCGCTTATGCGGCTTCCGCTGAGTTTGGGGTCCCTCTTATGGATCTCTCTGGCCTCTCTATCGAACAGATACCT
GACAAAATCGTTGACGAAAAGCTCATTCGCAAACACCACGCACTTCCTATATTCAAAAGAGGAAATCGACTCTTTCTGGC
GGTCGCAGATCCCACTAACTTGCAAGCGCTTGACGAGATCAAGTTTCATACCGGTCTTGGTACCGACGCCATTCTAGTGG
AAGAGGATAAGCTTTCTGCGTTAATTGAAAAATACTTGGACTCACATGAGCAACCTTTGGGAGACCTTGGAGATACCGAA
CTTGATGATATTGATATTGACGCTGGAGATGAACCAGACAGAGCTGACGACTCTGGGCCAGAGCAAGACGATGCGCCAAT
AGTTAAGTACATCAATAAGATGCTCCTTGATGCGATCAAAGGCGGGGCTTCAGACATACACTTTGAGCCTTACGAAAAGT
CATATCGTGTAAGGTTTAGGACAGACGGCATATTGAACGAAGTATCTAAGCCTTCTATAAAGCTAGCCGCAAAGTTAGCA
GCCCGCTTAAAAGTTATGTCTCAGCTCGATATATCTGAACGTAGGGTGCCTCAAGACGGCAGAATAAAAATGAAGCTTTC
GAAAACAAAGGCTATCGATTTTCGTGTCAACACTCTTCCTACCCTATGGGGAGAAAAAATCGTTTTAAGGATTCTTGACC
CAAGTAGCGCAAAACTGGGAATAGACGCATTAGGGTACGAAGAAGACCAGAAAAAGATGTATTTGGATGCTCTCGCGCAG
CCGCAAGGTATGATTCTAGTAACAGGACCTACCGGAAGTGGAAAAACAGTCTCTTTATATACAGGTCTAAATATACTCAA
TACAACAGAGCGAAATATTTCCACAGCAGAAGACCCAGTTGAGATAAACCTGGAAGGAGTAAACCAAGTAAATGTCAACA
CAAGAGTAGGCTTGGACTTTTCATCCGCTCTAAGAGCTTTCTTACGCCAAGACCCAGATGTAGTTATGGTTGGGGAGATT
CGGGATTTGGATACTGCCTCCATAGCAATCAAAGCCGCCCAGACTGGACACTTAGTCCTATCAACTCTCCATACCAATAG
TGCAGCGGAAACTCTAACTCGTTTAAGAAATATGGGAGTGCCAGCGTTTAACATAGCTACTTCTGTAAGCCTCATAATTG
CTCAACGATTAGCCAGAAGGTTATGCTCCGAATGCAAGGAGACCATTACTGTTCCAAATGATGTGCTAAAAGAGGAAGGC
TTCACAGACAAACAAATTGAAACAGGCTTTAAATTGTATAGGCCCAAAGGCTGTGATAAATGCAAGGATGGGTATAAGGG
ACGTGTTGGTATCTACGAGGTGGTAAGGATCACCCCTGCTATGCAAGAACTTATTATGAACGAAGCCAGTTCGCTAGAGT
TGGCGAAGCAAGCGCAAAAAGAAGGCTTCCGTAACCTCCGACAGTCTGCGCTCAGAAAGGTAATAGAAGGCGTAACCAGT
CTAGAAGAAGCAAATCGCGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.875

99.825

0.588
  pilB Acinetobacter baylyi ADP1

57.766

100

0.581
  pilB Legionella pneumophila strain ERS1305867

55.986

99.649

0.558
  pilB Vibrio parahaemolyticus RIMD 2210633

52.763

98.421

0.519
  pilB Vibrio campbellii strain DS40M4

51.056

99.649

0.509
  pilB Vibrio cholerae strain A1552

54.302

91.754

0.498
  pilF Neisseria gonorrhoeae MS11

47.276

99.825

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

40.848

91.053

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.722

93.333

0.361

Multiple sequence alignment