Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EUZ85_RS09225 Genome accession   NZ_CP035490
Coordinates   2060704..2061582 (-) Length   292 a.a.
NCBI ID   WP_127969020.1    Uniprot ID   -
Organism   Hahella sp. KA22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2055704..2066582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUZ85_RS31120 - 2057669..2058025 (+) 357 WP_256372771.1 PilZ domain-containing protein -
  EUZ85_RS09205 (EUZ85_09205) tsaA 2058015..2058731 (-) 717 WP_127969016.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  EUZ85_RS09210 (EUZ85_09210) - 2058741..2059610 (-) 870 WP_127969017.1 hypothetical protein -
  EUZ85_RS09215 (EUZ85_09215) yacG 2059832..2060068 (-) 237 WP_127969018.1 DNA gyrase inhibitor YacG -
  EUZ85_RS09220 (EUZ85_09220) coaE 2060058..2060684 (-) 627 WP_127969019.1 dephospho-CoA kinase -
  EUZ85_RS09225 (EUZ85_09225) pilD 2060704..2061582 (-) 879 WP_127969020.1 A24 family peptidase Machinery gene
  EUZ85_RS09230 (EUZ85_09230) pilC 2061649..2062869 (-) 1221 WP_127969021.1 type II secretion system F family protein Machinery gene
  EUZ85_RS09235 (EUZ85_09235) pilB 2062873..2064585 (-) 1713 WP_127969022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EUZ85_RS09240 (EUZ85_09240) pilE 2064906..2065349 (+) 444 WP_127969023.1 pilin Machinery gene

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 31827.07 Da        Isoelectric Point: 7.1060

>NTDB_id=342767 EUZ85_RS09225 WP_127969020.1 2060704..2061582(-) (pilD) [Hahella sp. KA22]
MLEIFSQFNHNPALLYTTVIVFGLVVGSFLNVVILRKPKMLEQEWRAQCAEIPGCDCPKTDEPLITLSKPDSTCPKCGHK
IRAWENIPVISYLFLGGKCSSCKTGISIRYPLVEIATALLAVIVVCYFKMSWAGLAAIGLSWTLLTLSLIDFDTQLLPDD
LTLPLLWAGLLINIQGIFAPLSEAVIGAAIGYLALWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWKMLPLIILLSSL
VGAVIGLAMIITLGRDKNIPIPFGPYLAIAGWIAVIWGNDIVSAYLGMYPAP

Nucleotide


Download         Length: 879 bp        

>NTDB_id=342767 EUZ85_RS09225 WP_127969020.1 2060704..2061582(-) (pilD) [Hahella sp. KA22]
ATGTTAGAAATTTTTAGTCAGTTCAATCATAACCCTGCGCTGCTTTATACCACGGTCATTGTTTTCGGCTTGGTTGTCGG
CAGCTTTTTGAATGTCGTGATTCTGCGAAAGCCGAAAATGCTCGAGCAGGAGTGGCGGGCTCAGTGCGCTGAAATTCCAG
GCTGCGACTGCCCTAAAACAGATGAACCCCTGATCACGCTTTCCAAACCCGATTCAACCTGCCCTAAGTGCGGACATAAA
ATCAGGGCCTGGGAAAATATTCCGGTAATCAGTTATTTGTTTCTTGGCGGCAAGTGCTCCTCGTGCAAAACGGGTATTTC
TATTCGCTATCCGCTAGTGGAAATCGCCACCGCTTTATTAGCGGTCATTGTCGTCTGCTATTTCAAGATGAGTTGGGCGG
GACTGGCGGCCATCGGCTTAAGCTGGACATTGCTTACTTTGTCATTGATTGACTTCGACACCCAGCTACTGCCTGATGAC
CTGACGCTGCCTCTGCTTTGGGCGGGATTGTTAATCAATATCCAAGGTATTTTCGCCCCTCTATCAGAAGCTGTGATTGG
CGCAGCCATTGGCTATTTGGCTTTGTGGTCGGTTTACCACTTATTCCGCCTTTTGACCGGCAAGGAAGGTATGGGTTACG
GCGACTTCAAGCTGCTTGCCGCTCTTGGGGCCTGGCTGGGCTGGAAAATGCTCCCGCTCATTATTCTGCTTTCATCACTG
GTTGGAGCCGTTATTGGCCTGGCGATGATTATTACCTTGGGTAGAGATAAAAATATCCCGATCCCATTCGGCCCCTATCT
GGCAATCGCCGGCTGGATAGCCGTGATCTGGGGAAATGATATCGTGTCCGCCTATTTGGGGATGTACCCTGCGCCTTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(140-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.025

96.233

0.51
  pilD Acinetobacter baumannii D1279779

51.916

98.288

0.51
  pilD Acinetobacter nosocomialis M2

51.568

98.288

0.507
  pilD Vibrio campbellii strain DS40M4

52.128

96.575

0.503
  pilD Neisseria gonorrhoeae MS11

51.136

90.411

0.462

Multiple sequence alignment