Detailed information NTG0342645

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EQU24_RS04470
• Genome accession: NZ_CP035467
• Coordinates: 934523..935584 (+)
• Length: 353 a.a.
• NCBI ID: WP_026130159.1
• Uniprot ID: A0A4P9UK75
• Organism: Methylotuvimicrobium buryatense strain 5GB1C
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 929523..940584

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQU24_RS04460 (EQU24_04460)	-	929715..931211 (+)	1497	WP_017840652.1	hypothetical protein	-
EQU24_RS04465 (EQU24_04465)	-	931317..933689 (-)	2373	WP_017840651.1	penicillin-binding protein 1A	-
EQU24_RS04470 (EQU24_04470)	pilM	934523..935584 (+)	1062	WP_026130159.1	pilus assembly protein PilM	Machinery gene
EQU24_RS04475 (EQU24_04475)	-	935584..936147 (+)	564	WP_017840649.1	PilN domain-containing protein	-
EQU24_RS04480 (EQU24_04480)	pilO	936147..936782 (+)	636	WP_017840648.1	type 4a pilus biogenesis protein PilO	-
EQU24_RS04485 (EQU24_04485)	-	936779..937378 (+)	600	WP_017840647.1	pilus assembly protein PilP	-
EQU24_RS04490 (EQU24_04490)	pilQ	937395..939716 (+)	2322	WP_017840646.1	type IV pilus secretin family protein	-
EQU24_RS23210	-	939794..940027 (+)	234	WP_017840645.1	shikimate kinase	-
EQU24_RS04500 (EQU24_04500)	aroK	940020..940538 (+)	519	WP_017840644.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38433.99 Da
• Isoelectric Point: 4.8020

>NTDB_id=342645 EQU24_RS04470 WP_026130159.1 934523..935584(+) (pilM) [Methylotuvimicrobium buryatense strain 5GB1C]
MNWFSRKNVPVVGIDISTAAVKLLELSKNGPRYRVESYAVAPLPQDAVIDKNFANVDVISDAIKQAIKQSGTKVKHACVA
VAGSAVMTKIITMPANYSDEEVYEQIMVEADQYVPYALDEVNLDFEVQGKNAKNSEMVDVLLVASRRENVEDRIEALEKA
GLKAKIIDVEAFAMENAFALISDQLPGSTEGQTIAISDIGSTMATLNVIANGRTIYTREQGFGGRQLTDEIQRRYGLSYE
EAGLAKRHGGLPDNYIIDVLEPFKKAMVQQIARSLQFFVSSSANRTIDSIILAGGCSSIPGVDKLVEQNLSIPCFIANPF
INMALSNRVKPQSLSSDAPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=342645 EQU24_RS04470 WP_026130159.1 934523..935584(+) (pilM) [Methylotuvimicrobium buryatense strain 5GB1C]
ATGAACTGGTTTAGTCGCAAAAATGTGCCTGTAGTCGGAATCGACATTAGCACGGCAGCCGTTAAGTTATTGGAATTATC
GAAAAACGGTCCTCGTTATCGAGTCGAAAGTTATGCTGTAGCGCCATTGCCGCAGGATGCCGTTATCGATAAAAATTTCG
CGAATGTCGATGTAATCTCTGATGCGATCAAACAAGCCATCAAACAATCCGGAACGAAAGTCAAACATGCCTGCGTCGCG
GTTGCGGGTTCGGCGGTCATGACCAAAATCATTACGATGCCCGCTAATTATTCGGACGAAGAAGTCTACGAACAAATCAT
GGTAGAGGCAGATCAATATGTGCCTTATGCTCTGGATGAGGTCAACCTGGATTTTGAAGTTCAAGGAAAAAACGCTAAAA
ACTCCGAAATGGTTGATGTGCTCTTGGTTGCATCCCGTCGTGAAAATGTCGAGGACCGCATCGAGGCCTTGGAAAAGGCT
GGTTTAAAAGCCAAAATCATTGATGTCGAAGCGTTTGCGATGGAAAACGCCTTTGCGCTTATCTCCGATCAATTGCCCGG
TTCAACCGAAGGGCAGACTATTGCTATCTCGGATATCGGTTCGACGATGGCGACTTTGAACGTCATTGCGAACGGTAGAA
CCATTTATACGCGCGAACAGGGCTTCGGCGGAAGGCAGTTGACCGATGAAATTCAACGACGCTACGGTTTGTCCTACGAA
GAAGCCGGTCTTGCCAAGCGGCACGGCGGTCTTCCGGATAATTATATTATTGACGTATTGGAGCCATTCAAGAAGGCGAT
GGTTCAGCAAATCGCCCGATCGCTCCAATTTTTTGTTTCATCGAGCGCCAACCGAACGATCGATTCGATTATTTTGGCGG
GTGGTTGTTCCTCGATACCCGGTGTCGATAAGTTGGTCGAGCAGAACTTGAGTATTCCCTGTTTTATTGCCAATCCGTTT
ATCAATATGGCTTTATCGAATCGAGTGAAACCGCAAAGTTTAAGTAGCGATGCGCCGGCTATGATGATTGCCTGCGGTCT
TGCGCTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

3D structure

• AlphaFold DB: A0A4P9UK75

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.558	100	0.516
comM	Acinetobacter nosocomialis M2	51.275	100	0.513
comM	Acinetobacter baylyi ADP1	48.442	100	0.484
pilM	Legionella pneumophila strain ERS1305867	40.793	100	0.408