Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   ETT53_RS00815 Genome accession   NZ_CP035446
Coordinates   118368..118802 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm68.2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 113368..123802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT53_RS00785 (ETT53_00785) - 115037..115402 (+) 366 WP_002986560.1 DUF1033 family protein -
  ETT53_RS00790 (ETT53_00790) comYA 115495..116433 (+) 939 WP_111684478.1 competence type IV pilus ATPase ComGA Machinery gene
  ETT53_RS00795 (ETT53_00795) comYB 116369..117403 (+) 1035 WP_261752453.1 competence type IV pilus assembly protein ComGB Machinery gene
  ETT53_RS00800 (ETT53_00800) comYC 117405..117731 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  ETT53_RS00805 (ETT53_00805) comGD 117706..118134 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  ETT53_RS00810 (ETT53_00810) comGE 118091..118375 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  ETT53_RS00815 (ETT53_00815) comYF 118368..118802 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  ETT53_RS00820 (ETT53_00820) comGG 118786..119112 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  ETT53_RS00825 (ETT53_00825) comYH 119210..120163 (+) 954 WP_038433850.1 class I SAM-dependent methyltransferase Machinery gene
  ETT53_RS00830 (ETT53_00830) - 120222..121418 (+) 1197 WP_129321437.1 acetate kinase -
  ETT53_RS00835 (ETT53_00835) - 121605..121913 (+) 309 Protein_116 hypothetical protein -
  ETT53_RS00840 (ETT53_00840) proC 121996..122766 (-) 771 WP_038433852.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=342001 ETT53_RS00815 WP_002992738.1 118368..118802(+) (comYF) [Streptococcus pyogenes strain emm68.2]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=342001 ETT53_RS00815 WP_002992738.1 118368..118802(+) (comYF) [Streptococcus pyogenes strain emm68.2]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment