Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ETK69_RS09925 Genome accession   NZ_CP035410
Coordinates   1962797..1963231 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain SRCM103616     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1957797..1968231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK69_RS09920 (ETK69_09920) - 1961297..1962508 (-) 1212 WP_025649439.1 cytochrome P450 -
  ETK69_RS09925 (ETK69_09925) nucA/comI 1962797..1963231 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ETK69_RS09930 (ETK69_09930) - 1963291..1964046 (+) 756 WP_022553315.1 YoaK family protein -
  ETK69_RS09935 (ETK69_09935) - 1964080..1964442 (-) 363 WP_007410383.1 hypothetical protein -
  ETK69_RS09940 (ETK69_09940) - 1964634..1965962 (-) 1329 WP_094295768.1 S8 family peptidase -
  ETK69_RS09945 (ETK69_09945) - 1966141..1966374 (+) 234 WP_106067946.1 hypothetical protein -
  ETK69_RS09950 (ETK69_09950) - 1966630..1967337 (+) 708 WP_014417828.1 poly-gamma-glutamate hydrolase family protein -
  ETK69_RS09955 (ETK69_09955) - 1967397..1967849 (+) 453 WP_014305039.1 OsmC family protein -
  ETK69_RS09960 (ETK69_09960) - 1967863..1968216 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=340635 ETK69_RS09925 WP_007611576.1 1962797..1963231(+) (nucA/comI) [Bacillus velezensis strain SRCM103616]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=340635 ETK69_RS09925 WP_007611576.1 1962797..1963231(+) (nucA/comI) [Bacillus velezensis strain SRCM103616]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment