Detailed information NTG0339795

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ES967_RS12820
• Genome accession: NZ_CP035394
• Coordinates: 2485953..2486570 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain SRCM103696
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2480953..2491570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ES967_RS12790 (ES967_12790)	rpsT	2481254..2481520 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ES967_RS12795 (ES967_12795)	holA	2481535..2482578 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
ES967_RS12800 (ES967_12800)	-	2482618..2482767 (-)	150	WP_003229985.1	hypothetical protein	-
ES967_RS12805 (ES967_12805)	yqzM	2482808..2482942 (+)	135	WP_003229983.1	YqzM family protein	-
ES967_RS12810 (ES967_12810)	comEC	2482983..2485313 (-)	2331	WP_069704087.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ES967_RS12815 (ES967_12815)	comEB	2485317..2485886 (-)	570	WP_003229978.1	ComE operon protein 2	-
ES967_RS12820 (ES967_12820)	comEA	2485953..2486570 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
ES967_RS12825 (ES967_12825)	comER	2486654..2487475 (+)	822	WP_014480313.1	late competence protein ComER	-
ES967_RS12830 (ES967_12830)	yqeM	2487541..2488284 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
ES967_RS12835 (ES967_12835)	rsfS	2488281..2488637 (-)	357	WP_014480315.1	ribosome silencing factor	-
ES967_RS12840 (ES967_12840)	yqeK	2488655..2489215 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ES967_RS12845 (ES967_12845)	nadD	2489205..2489774 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ES967_RS12850 (ES967_12850)	yhbY	2489786..2490076 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ES967_RS12855 (ES967_12855)	aroE	2490070..2490912 (-)	843	WP_041351695.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=339795 ES967_RS12820 WP_004398514.1 2485953..2486570(-) (comEA) [Bacillus subtilis strain SRCM103696]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=339795 ES967_RS12820 WP_004398514.1 2485953..2486570(-) (comEA) [Bacillus subtilis strain SRCM103696]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39