Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ES966_RS12600 Genome accession   NZ_CP035393
Coordinates   2552428..2553498 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain SRCM103691     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2547428..2558498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES966_RS12550 (ES966_12550) - 2547487..2548071 (-) 585 WP_012117977.1 signal peptidase I -
  ES966_RS12555 (ES966_12555) tapA 2548043..2548714 (-) 672 WP_025649852.1 amyloid fiber anchoring/assembly protein TapA -
  ES966_RS12560 (ES966_12560) - 2548973..2549302 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  ES966_RS12565 (ES966_12565) - 2549343..2549522 (-) 180 WP_003153093.1 YqzE family protein -
  ES966_RS12570 (ES966_12570) comGG 2549579..2549956 (-) 378 WP_025649851.1 competence type IV pilus minor pilin ComGG Machinery gene
  ES966_RS12575 (ES966_12575) comGF 2549957..2550352 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  ES966_RS12580 (ES966_12580) comGE 2550366..2550680 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  ES966_RS12585 (ES966_12585) comGD 2550664..2551101 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  ES966_RS12590 (ES966_12590) comGC 2551091..2551399 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  ES966_RS12595 (ES966_12595) comGB 2551404..2552441 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  ES966_RS12600 (ES966_12600) comGA 2552428..2553498 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  ES966_RS12605 (ES966_12605) - 2553654..2554642 (-) 989 Protein_2430 magnesium transporter CorA family protein -
  ES966_RS12610 (ES966_12610) - 2554788..2556089 (+) 1302 WP_017418142.1 hemolysin family protein -
  ES966_RS12615 (ES966_12615) - 2556127..2556957 (-) 831 WP_017418143.1 STAS domain-containing protein -
  ES966_RS12625 (ES966_12625) - 2557164..2557544 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ES966_RS12630 (ES966_12630) - 2557781..2558025 (+) 245 Protein_2434 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=339709 ES966_RS12600 WP_012117985.1 2552428..2553498(-) (comGA) [Bacillus velezensis strain SRCM103691]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=339709 ES966_RS12600 WP_012117985.1 2552428..2553498(-) (comGA) [Bacillus velezensis strain SRCM103691]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374

Multiple sequence alignment