Detailed information NTG0339056

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EO087_RS09750
• Genome accession: NZ_CP035300
• Coordinates: 2083866..2084924 (-)
• Length: 352 a.a.
• NCBI ID: WP_128898698.1
• Uniprot ID: A0A410UIW9
• Organism: Dyella sp. M7H15-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2078866..2089924

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EO087_RS09730 (EO087_09730)	pilQ	2079723..2081894 (-)	2172	WP_128898694.1	type IV pilus secretin family protein	-
EO087_RS09735 (EO087_09735)	-	2081949..2082482 (-)	534	WP_128898695.1	pilus assembly protein PilP	-
EO087_RS09740 (EO087_09740)	pilO	2082479..2083144 (-)	666	WP_128898696.1	type 4a pilus biogenesis protein PilO	-
EO087_RS09745 (EO087_09745)	-	2083144..2083866 (-)	723	WP_128898697.1	PilN domain-containing protein	-
EO087_RS09750 (EO087_09750)	pilM	2083866..2084924 (-)	1059	WP_128898698.1	pilus assembly protein PilM	Machinery gene
EO087_RS09755 (EO087_09755)	-	2085354..2087873 (+)	2520	WP_128898699.1	penicillin-binding protein 1A	-
EO087_RS09760 (EO087_09760)	-	2088014..2088650 (+)	637	Protein_1904	hypothetical protein	-
EO087_RS09765 (EO087_09765)	-	2088662..2089054 (+)	393	WP_128899885.1	MAPEG family protein	-
EO087_RS09770 (EO087_09770)	-	2089066..2089881 (+)	816	WP_164931809.1	type II CAAX endopeptidase family protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 37981.66 Da
• Isoelectric Point: 4.5729

>NTDB_id=339056 EO087_RS09750 WP_128898698.1 2083866..2084924(-) (pilM) [Dyella sp. M7H15-1]
MGLFTPKKPPLVGVDISSTAVKLLQLSQTGGRYRVEHYAIEPLPPNAVVEKNIVEVEAVGEAVRRALTRSGAKIKHAAAA
VAGSAVITRIVPMAVELSDEDLEGQIQVEANQYIPYPIEEVSLDFEVLGPVRDSPEMNNVLLAASRTENVDMRVAALDLG
GLAAQVIDVEAFAMENAFTLIADQLNVGRDALVAVVDIGATMTTLSVLKNQRTIYSREQVFGGKQLTDEIMRRYGLSYEE
AGRAKRKGGLPESYETETLLPFKESLVQQISRLLQFFYAGSEFSKVDQVVLAGGCASIEGISAMLEEQLGLPCIVANPLA
RMSLSSRVQAQSLTQDAPALMIAVGLAMRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=339056 EO087_RS09750 WP_128898698.1 2083866..2084924(-) (pilM) [Dyella sp. M7H15-1]
GTGGGGCTCTTTACACCCAAGAAGCCGCCGCTTGTTGGTGTCGATATCAGTTCGACCGCCGTCAAGCTGCTGCAGCTCAG
CCAGACTGGAGGCCGCTATCGCGTGGAGCACTACGCGATCGAGCCGCTGCCCCCCAATGCCGTCGTCGAGAAAAACATCG
TCGAGGTCGAGGCGGTCGGTGAGGCGGTACGCCGCGCGCTGACGCGCTCCGGCGCCAAGATCAAGCATGCGGCGGCGGCA
GTAGCCGGCTCGGCCGTGATTACGCGCATTGTGCCGATGGCGGTGGAGCTTTCTGACGAGGATCTTGAGGGTCAGATCCA
GGTCGAGGCCAACCAGTACATCCCGTATCCGATCGAGGAAGTCAGTCTCGATTTCGAGGTGCTGGGTCCGGTGCGCGACA
GTCCCGAAATGAACAACGTGCTGTTGGCTGCGTCGCGCACGGAGAATGTCGATATGCGCGTGGCCGCGCTCGATCTGGGC
GGCTTGGCCGCGCAGGTGATCGACGTGGAAGCCTTCGCGATGGAGAACGCTTTCACGCTGATCGCCGATCAGTTGAATGT
CGGCCGCGATGCGCTGGTGGCAGTGGTCGATATCGGCGCCACCATGACCACGCTCTCCGTGCTCAAGAACCAGCGCACCA
TCTATTCGCGCGAGCAAGTGTTCGGTGGCAAGCAGCTCACCGACGAAATCATGCGCCGCTATGGCCTTTCTTACGAGGAA
GCCGGTCGCGCCAAGCGCAAAGGTGGTCTGCCGGAATCCTACGAGACCGAAACATTGCTGCCGTTCAAGGAGTCGCTGGT
GCAGCAGATCAGTCGCTTGCTGCAGTTCTTCTACGCCGGTAGCGAATTCAGCAAGGTCGACCAGGTCGTGCTGGCGGGTG
GTTGTGCGTCGATCGAAGGCATCAGCGCGATGCTGGAAGAGCAGCTTGGCCTGCCTTGCATCGTCGCCAATCCGCTGGCC
CGCATGTCGCTGTCCAGCCGGGTGCAGGCACAGTCGCTCACCCAGGATGCACCGGCTCTGATGATCGCGGTCGGACTCGC
CATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-351)

3D structure

• AlphaFold DB: A0A410UIW9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	52.011	98.864	0.514
comM	Acinetobacter nosocomialis M2	51.724	98.864	0.511
comM	Acinetobacter baylyi ADP1	49.429	99.432	0.491
pilM	Legionella pneumophila strain ERS1305867	43.343	100	0.435