Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   EQ030_RS00185 Genome accession   NZ_CP035294
Coordinates   40908..42536 (-) Length   542 a.a.
NCBI ID   WP_011302038.1    Uniprot ID   -
Organism   Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292 strain ATCC 15305     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 41077..58149 40908..42536 flank -1459


Gene organization within MGE regions


Location: 40908..58149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQ030_RS00185 (EQ030_00185) ccrB 40908..42536 (-) 1629 WP_011302038.1 recombinase family protein Machinery gene
  EQ030_RS00190 (EQ030_00190) ccrA 42557..43906 (-) 1350 WP_011302039.1 recombinase family protein Machinery gene
  EQ030_RS00195 (EQ030_00195) - 44084..44341 (-) 258 WP_011302040.1 hypothetical protein -
  EQ030_RS00200 (EQ030_00200) - 44406..46196 (-) 1791 WP_011302041.1 DUF927 domain-containing protein -
  EQ030_RS00205 (EQ030_00205) - 46322..47749 (-) 1428 WP_011302042.1 DUF6038 family protein -
  EQ030_RS00210 (EQ030_00210) - 48176..49369 (+) 1194 WP_011302043.1 PD-(D/E)XK nuclease family protein -
  EQ030_RS00215 (EQ030_00215) - 49658..51397 (+) 1740 WP_011302044.1 hypothetical protein -
  EQ030_RS00220 (EQ030_00220) - 51439..52914 (+) 1476 WP_011302045.1 DUF6038 family protein -
  EQ030_RS00225 (EQ030_00225) - 53141..54241 (+) 1101 WP_011302046.1 DNA polymerase -
  EQ030_RS00230 (EQ030_00230) - 54234..54602 (+) 369 WP_041784734.1 hypothetical protein -
  EQ030_RS00235 (EQ030_00235) - 54602..56242 (+) 1641 WP_011302047.1 DUF5906 domain-containing protein -
  EQ030_RS00240 (EQ030_00240) - 56467..58149 (+) 1683 WP_011302048.1 recombinase family protein -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62354.36 Da        Isoelectric Point: 9.6600

>NTDB_id=339005 EQ030_RS00185 WP_011302038.1 40908..42536(-) (ccrB) [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292 strain ATCC 15305]
MQQLKQKRVGIYVRVSTEMQSAEGYSIDGQINQIKEYCNFHHFEVKDIYADRGISGKSMNRPALQRMLHDANKGHIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNTILENIYTGQHQRALEGYYQGN
LPLGYNNIPDNKKDLMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGNPFSISAVTYILSNPFYIGKIQFAK
YKDWNDKRRKGLNDKPVIAEGKHAPIIGKDLWDKVQARKKQVSEKPQVHGKGTNILTGLISCPQCSASMSASTTVNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEKYVMDQILEIVKSDKVIKQVVERVNQEHQIDMAALNHDIAYKQQQYD
EVQIKLDNLIKTLEDNPDLTSVLKNTIHQYEKQLNDITTQINQLKQQQNQEKPSYDTKQIAALLQQIFQNVESMDKVQLK
ALYLTVIDRIDIRKDGNHKKQFYVTLKLNNEIIKQLFNDTPLDEVLLSTSSLFLPQTLFLQI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=339005 EQ030_RS00185 WP_011302038.1 40908..42536(-) (ccrB) [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC 7292 strain ATCC 15305]
ATGCAACAACTCAAACAAAAACGTGTCGGTATCTATGTCCGCGTATCAACAGAAATGCAAAGTGCAGAGGGTTATAGTAT
CGACGGTCAAATCAATCAAATCAAAGAATATTGTAACTTCCATCATTTCGAAGTCAAAGATATATACGCTGATCGTGGTA
TTTCAGGAAAGTCTATGAACCGCCCTGCACTTCAACGTATGTTACATGATGCGAATAAAGGTCATATTGATTGTGTTATG
GTGTATAAAACAAATCGACTCGCACGTAATACATCAGACTTACTCAAAATTGTCGAAGATTTGCATAAACAAAATGTCGA
ATTTTTCAGTTTGTCTGAGCGTATGGAAGTCAACACTTCTTCTGGTAAACTCATGTTACAAATACTTGCAAGTTTTTCCG
AATTTGAAAGAAATACCATTTTAGAGAATATTTACACCGGACAACATCAAAGAGCCTTAGAAGGCTATTACCAAGGTAAT
CTTCCTTTAGGATATAATAACATACCAGATAATAAAAAAGATTTGATGATTAATCAGCATGAAGCTAATATTGTGAAATA
TATTTTTGAATCTTACGCCAAAGGTCATGGTTATCGTAAAATTGCCAACGCACTCAATCATAAAGGTTATGTCACTAAGA
AAGGTAATCCATTTAGTATTTCAGCTGTTACTTATATTCTCTCAAACCCATTCTATATTGGTAAAATTCAATTCGCTAAA
TACAAAGATTGGAATGATAAACGTCGTAAAGGCTTGAATGATAAGCCAGTAATCGCTGAAGGTAAGCACGCCCCTATTAT
TGGCAAAGACTTATGGGATAAAGTCCAAGCACGTAAAAAACAAGTAAGTGAAAAACCACAAGTTCATGGTAAAGGAACCA
ATATTTTAACTGGATTAATTTCGTGTCCCCAATGTTCTGCTTCAATGTCTGCGAGCACAACAGTTAATACACTTAAAGAT
GGTACTAAAAAACGCATTCGCTACTATTCGTGTAGTAACTTTCGTAATAAAGGGTCAAAAGTATGTTCTGCGAATAGTGT
CAGAGCCGATGTAATTGAAAAATATGTTATGGATCAAATACTCGAAATTGTTAAAAGCGACAAGGTTATCAAGCAAGTCG
TTGAGCGTGTCAATCAAGAACATCAAATTGATATGGCTGCACTCAATCATGATATCGCATATAAGCAACAACAATATGAT
GAGGTCCAAATCAAATTAGATAACTTGATAAAAACGCTTGAAGATAATCCAGACTTAACATCTGTGCTTAAAAATACGAT
TCATCAGTATGAAAAACAACTCAATGACATCACAACTCAAATCAATCAACTTAAGCAACAACAAAATCAAGAGAAACCAT
CTTATGATACGAAACAAATCGCTGCCCTATTACAACAAATATTTCAAAACGTAGAATCAATGGATAAAGTACAACTCAAA
GCATTATACCTTACGGTCATTGATCGTATCGATATTCGTAAAGACGGTAATCATAAAAAACAGTTCTACGTTACACTCAA
ACTCAATAATGAAATTATTAAACAACTTTTCAATGATACCCCACTCGACGAAGTGCTCCTCAGCACTTCGTCTTTATTTT
TGCCTCAAACACTCTTTCTTCAAATCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-155)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(302-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

83.764

100

0.838

Multiple sequence alignment