Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   F0316_RS00665 Genome accession   NZ_CP043554
Coordinates   129817..131328 (+) Length   503 a.a.
NCBI ID   WP_000138174.1    Uniprot ID   P37093
Organism   Vibrio cholerae strain RFB16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 124817..136328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0316_RS00645 (F0316_00645) hslO 125317..126192 (-) 876 WP_001911276.1 Hsp33 family molecular chaperone HslO -
  F0316_RS00650 (F0316_00650) hslR 126226..126603 (-) 378 WP_000098852.1 ribosome-associated heat shock protein Hsp15 -
  F0316_RS00655 (F0316_00655) gspC 126833..127750 (+) 918 WP_000394675.1 type II secretion system protein GspC -
  F0316_RS00660 (F0316_00660) gspD 127796..129820 (+) 2025 WP_000878557.1 type II secretion system secretin GspD -
  F0316_RS00665 (F0316_00665) pilB/pilB1 129817..131328 (+) 1512 WP_000138174.1 type II secretion system ATPase GspE Machinery gene
  F0316_RS00670 (F0316_00670) gspF 131328..132548 (+) 1221 WP_000718695.1 type II secretion system inner membrane protein GspF -
  F0316_RS00675 (F0316_00675) gspG 132589..133029 (+) 441 WP_000738789.1 type II secretion system major pseudopilin GspG -
  F0316_RS00680 (F0316_00680) gspH 133063..133647 (+) 585 WP_095471498.1 type II secretion system minor pseudopilin GspH -
  F0316_RS00685 (F0316_00685) gspI 133637..133990 (+) 354 WP_000837699.1 type II secretion system minor pseudopilin GspI -
  F0316_RS00690 (F0316_00690) gspJ 133977..134642 (+) 666 WP_149560143.1 type II secretion system minor pseudopilin GspJ -
  F0316_RS00695 (F0316_00695) gspK 134632..135642 (+) 1011 WP_001201881.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 56358.58 Da        Isoelectric Point: 6.2107

>NTDB_id=338338 F0316_RS00665 WP_000138174.1 129817..131328(+) (pilB/pilB1) [Vibrio cholerae strain RFB16]
MTEMVISPAERQSIRRLPFSFANRFKLVLDWNEDFSQASIYYLAPLSMEALVETKRVVKHAFQLIELSQAEFESKLTQVY
QRDSSEARQLMEDIGADSDDFFSLAEELPQNEDLLESEDDAPIIKLINAMLGEAIKEGASDIHIETFEKTLSIRFRVDGV
LREVLAPSRKLSSLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLG
MTAHNHDNFRRLIKRPHGIILVTGPTGSGKSTTLYAGLQELNSSERNILTVEDPIEFDIDGIGQTQVNPRVDMTFARGLR
AILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLISSSLLGVLAQRLVRTLCPDC
KEPYEADKEQRKLFDSKKKEPLILYRATGCPKCNHKGYRGRTGIHELLLVDDALQELIHSEAGEQAMEKHIRATTPSIRD
DGLDKVRQGITSLEEVMRVTKES

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=338338 F0316_RS00665 WP_000138174.1 129817..131328(+) (pilB/pilB1) [Vibrio cholerae strain RFB16]
ATGACCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTT
GGTGCTGGATTGGAATGAGGATTTCTCCCAAGCCAGCATTTATTACTTAGCGCCATTGTCGATGGAGGCGCTCGTCGAAA
CCAAGCGGGTGGTCAAGCACGCTTTCCAACTGATTGAGCTCTCTCAAGCGGAGTTTGAAAGCAAGCTAACCCAAGTCTAT
CAGCGTGATTCTTCAGAAGCGCGTCAATTGATGGAAGACATTGGTGCCGACAGTGATGACTTCTTCTCACTGGCGGAAGA
GCTGCCTCAAAACGAAGATTTGCTAGAAAGTGAAGATGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAG
CGATCAAAGAAGGCGCGTCAGATATTCACATTGAAACTTTTGAAAAAACACTGTCGATCCGTTTTCGAGTCGATGGCGTA
CTGCGCGAAGTACTGGCACCCAGCCGCAAACTCTCATCGCTGCTCGTTTCACGGGTCAAGGTGATGGCCAAGCTCGACAT
TGCCGAAAAACGTGTGCCGCAAGATGGCCGTATTTCGCTGCGTATCGGTGGCCGCGCGGTCGATGTGCGGGTTTCAACCA
TGCCTTCATCACATGGTGAGCGAGTGGTAATGCGTTTGCTGGATAAAAACGCGACGCGCCTTGATCTGCACAGTTTGGGT
ATGACGGCGCATAACCATGATAACTTCCGCCGTTTGATTAAGCGGCCGCATGGGATCATTCTGGTGACTGGCCCAACCGG
TTCGGGTAAATCGACCACCTTGTACGCGGGTTTGCAAGAGCTCAACAGCAGCGAGCGCAACATTTTAACCGTAGAAGATC
CGATCGAATTTGATATTGATGGTATCGGTCAGACCCAAGTCAACCCGAGGGTAGACATGACCTTCGCGCGGGGGCTACGT
GCTATTTTACGTCAAGACCCCGATGTGGTGATGGTGGGGGAAATTCGTGATTTGGAAACGGCGCAAATTGCGGTGCAAGC
CTCGCTGACCGGCCACTTAGTGATGTCGACTCTGCACACCAACACGGCCGTTGGGGCGGTGACTCGGCTGCGCGATATGG
GCATCGAGCCTTTTTTGATCTCCTCTTCACTGCTCGGTGTTCTGGCTCAGCGCTTGGTGCGCACCTTATGCCCAGATTGC
AAAGAGCCTTACGAGGCGGACAAAGAGCAGCGCAAACTGTTTGATAGCAAGAAAAAAGAACCGCTGATCCTTTATCGCGC
CACGGGCTGCCCTAAATGTAACCACAAAGGTTACCGTGGCCGAACCGGTATCCACGAGCTGCTGCTGGTGGATGATGCGT
TGCAGGAGCTGATCCATAGCGAGGCGGGCGAACAGGCGATGGAGAAACACATTCGCGCGACCACGCCGAGCATTCGTGAT
GATGGTCTAGATAAAGTGCGCCAAGGCATTACTTCGCTAGAAGAAGTGATGCGGGTGACTAAGGAGTCCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1P9R
  PDB 1P9W
  PDB 2BH1
  PDB 4KSR
  PDB 4KSS

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

41.412

100

0.431
  pilB Legionella pneumophila strain ERS1305867

47.126

86.481

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

44.619

88.668

0.396
  pilB Vibrio campbellii strain DS40M4

44.118

87.873

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.745

77.932

0.388
  pilB Vibrio cholerae strain A1552

48.485

78.728

0.382
  pilF Thermus thermophilus HB27

47.761

79.92

0.382
  pilB Acinetobacter baumannii D1279779

46.21

81.312

0.376

Multiple sequence alignment