Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   MGAS2221_RS00665 Genome accession   NZ_CP043530
Coordinates   104290..104718 (+) Length   142 a.a.
NCBI ID   WP_002986548.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain MGAS2221     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99290..109718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MGAS2221_RS00645 (MGAS2221_0130) - 101621..101986 (+) 366 WP_002986560.1 DUF1033 family protein -
  MGAS2221_RS00650 (MGAS2221_0131) comGA 102079..103017 (+) 939 WP_010921798.1 competence type IV pilus ATPase ComGA Machinery gene
  MGAS2221_RS00655 (MGAS2221_0132) comGB 102953..103987 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  MGAS2221_RS00660 (MGAS2221_0133) comGC 103989..104315 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  MGAS2221_RS00665 (MGAS2221_0134) comGD 104290..104718 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD Machinery gene
  MGAS2221_RS00670 (MGAS2221_0135) comGE 104675..104959 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE Machinery gene
  MGAS2221_RS00675 (MGAS2221_0136) comGF 104952..105386 (+) 435 WP_010921800.1 competence type IV pilus minor pilin ComGF Machinery gene
  MGAS2221_RS00680 (MGAS2221_0137) comGG 105370..105696 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  MGAS2221_RS00685 (MGAS2221_0138) comGH 105794..106747 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  MGAS2221_RS00690 (MGAS2221_0139) - 106806..108002 (+) 1197 WP_010921803.1 acetate kinase -
  MGAS2221_RS00695 - 108189..108497 (+) 309 WP_010921804.1 hypothetical protein -
  MGAS2221_RS00700 (MGAS2221_0140) proC 108580..109350 (-) 771 WP_010921805.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 16310.04 Da        Isoelectric Point: 10.3481

>NTDB_id=338093 MGAS2221_RS00665 WP_002986548.1 104290..104718(+) (comGD) [Streptococcus pyogenes strain MGAS2221]
MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEHLFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTE
GNSLTVPKSITVNHPYRLVIDQMGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP

Nucleotide


Download         Length: 429 bp        

>NTDB_id=338093 MGAS2221_RS00665 WP_002986548.1 104290..104718(+) (comGD) [Streptococcus pyogenes strain MGAS2221]
ATGAAAAAGCCCGTCTTAGCAATTAAAGCTTTTACTTTGCTTGAAACTTTACTAAGTTTGTCAGTGATGAGTTTCATTAT
ATTGGGATTGTCTGTACCAGTGACAAAAAGCTATCAAAAAGTAGAAGAGCATCTGTTTTTTAGCCATTTTGAACATTTGT
ATCGACATCAGCAAAAGTTAGCCATATTACAGCAAAAACAGCGCGTCTTGGATATTTCTTCAACAAAAATTGTTACAGAA
GGTAATAGCCTAACAGTTCCTAAATCAATAACAGTGAATCATCCTTATCGGTTAGTTATTGATCAGATGGGAGGCAACCA
TTCCTTAGCTAAAATTATTTTTGACATGACTGATCGACGCTTTAAATACCAATTTTATCTAGGGAGTGGGAATTATCAAA
AAACAAGTCAAAGCTTACATAGCCCTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Streptococcus mutans UA159

45.736

90.845

0.415
  comGD Streptococcus mutans UA140

45.736

90.845

0.415
  comGD/cglD Streptococcus mitis NCTC 12261

40.441

95.775

0.387
  comGD Streptococcus gordonii str. Challis substr. CH1

40.458

92.254

0.373
  comGD/cglD Streptococcus mitis SK321

39.695

92.254

0.366
  comGD/cglD Streptococcus pneumoniae Rx1

39.695

92.254

0.366
  comGD/cglD Streptococcus pneumoniae D39

39.695

92.254

0.366
  comGD/cglD Streptococcus pneumoniae R6

39.695

92.254

0.366

Multiple sequence alignment