Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EQW70_RS19070 Genome accession   NZ_CP035226
Coordinates   3612402..3613793 (-) Length   463 a.a.
NCBI ID   WP_128740769.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103517     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3607402..3618793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQW70_RS19035 (EQW70_19035) flgL 3607573..3608469 (-) 897 WP_014481046.1 flagellar hook-associated protein FlgL -
  EQW70_RS19040 (EQW70_19040) flgK 3608480..3610003 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  EQW70_RS19045 (EQW70_19045) flgN 3610022..3610504 (-) 483 WP_014481048.1 flagellar protein FlgN -
  EQW70_RS19050 (EQW70_19050) flgM 3610520..3610786 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  EQW70_RS19055 (EQW70_19055) yvyF 3610867..3611286 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  EQW70_RS19060 (EQW70_19060) comFC 3611360..3612082 (-) 723 WP_124047882.1 comF operon protein ComFC Machinery gene
  EQW70_RS19065 (EQW70_19065) comFB 3612046..3612342 (-) 297 WP_003227989.1 late competence protein ComFB -
  EQW70_RS19070 (EQW70_19070) comFA 3612402..3613793 (-) 1392 WP_128740769.1 ATP-dependent helicase ComFA Machinery gene
  EQW70_RS19075 (EQW70_19075) fakBA 3613901..3614746 (-) 846 WP_003227986.1 DegV family protein -
  EQW70_RS19080 (EQW70_19080) degU 3614844..3615533 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  EQW70_RS19085 (EQW70_19085) degS 3615616..3616773 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  EQW70_RS19090 (EQW70_19090) yvyE 3616990..3617643 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52533.45 Da        Isoelectric Point: 9.7976

>NTDB_id=336247 EQW70_RS19070 WP_128740769.1 3612402..3613793(-) (comFA) [Bacillus subtilis strain SRCM103517]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVSKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=336247 EQW70_RS19070 WP_128740769.1 3612402..3613793(-) (comFA) [Bacillus subtilis strain SRCM103517]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGAAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAATTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGGGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCTCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

97.84

100

0.978

Multiple sequence alignment