Detailed information NTG0335728

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EQ841_RS14595
• Genome accession: NZ_CP035186
• Coordinates: 3011112..3012230 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 11A1213CRGN008
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3006112..3017230

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQ841_RS14585 (EQ841_14585)	-	3009228..3009920 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
EQ841_RS14590 (EQ841_14590)	pilT	3010047..3011084 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EQ841_RS14595 (EQ841_14595)	pilU	3011112..3012230 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EQ841_RS14600 (EQ841_14600)	fur	3012300..3012737 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EQ841_RS14605 (EQ841_14605)	-	3012849..3013247 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EQ841_RS14610 (EQ841_14610)	-	3013302..3013625 (-)	324	WP_000442587.1	RnfH family protein	-
EQ841_RS14615 (EQ841_14615)	-	3013625..3014692 (-)	1068	WP_002019572.1	hypothetical protein	-
EQ841_RS14625 (EQ841_14625)	-	3014845..3015300 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EQ841_RS14630 (EQ841_14630)	-	3015553..3016401 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=335728 EQ841_RS14595 WP_000347039.1 3011112..3012230(+) (pilU) [Acinetobacter baumannii strain 11A1213CRGN008]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=335728 EQ841_RS14595 WP_000347039.1 3011112..3012230(+) (pilU) [Acinetobacter baumannii strain 11A1213CRGN008]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395