Detailed information NTG0335676

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EQ842_RS14530
• Genome accession: NZ_CP035185
• Coordinates: 3000576..3001694 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 11A1213CRGN055
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2995576..3006694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQ842_RS14520 (EQ842_14520)	-	2998692..2999384 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
EQ842_RS14525 (EQ842_14525)	pilT	2999511..3000548 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EQ842_RS14530 (EQ842_14530)	pilU	3000576..3001694 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EQ842_RS14535 (EQ842_14535)	fur	3001764..3002201 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EQ842_RS14540 (EQ842_14540)	-	3002313..3002711 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EQ842_RS14545 (EQ842_14545)	-	3002766..3003089 (-)	324	WP_000442587.1	RnfH family protein	-
EQ842_RS14550 (EQ842_14550)	-	3003089..3004156 (-)	1068	WP_002019572.1	hypothetical protein	-
EQ842_RS14560 (EQ842_14560)	-	3004309..3004764 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EQ842_RS14565 (EQ842_14565)	-	3005017..3005865 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=335676 EQ842_RS14530 WP_000347039.1 3000576..3001694(+) (pilU) [Acinetobacter baumannii strain 11A1213CRGN055]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=335676 EQ842_RS14530 WP_000347039.1 3000576..3001694(+) (pilU) [Acinetobacter baumannii strain 11A1213CRGN055]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395