Detailed information NTG0335130

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQI27_RS12995
• Genome accession: NZ_CP035163
• Coordinates: 2512905..2513522 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain SRCM103923
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2507905..2518522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQI27_RS12965 (EQI27_12965)	rpsT	2508214..2508480 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQI27_RS12970 (EQI27_12970)	holA	2508495..2509538 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
EQI27_RS12975 (EQI27_12975)	-	2509578..2509727 (-)	150	WP_003229985.1	hypothetical protein	-
EQI27_RS12980 (EQI27_12980)	yqzM	2509768..2509902 (+)	135	WP_003229983.1	YqzM family protein	-
EQI27_RS12985 (EQI27_12985)	comEC	2509942..2512272 (-)	2331	WP_074794436.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQI27_RS12990 (EQI27_12990)	comEB	2512269..2512838 (-)	570	WP_015714288.1	ComE operon protein 2	-
EQI27_RS12995 (EQI27_12995)	comEA	2512905..2513522 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
EQI27_RS13000 (EQI27_13000)	comER	2513606..2514427 (+)	822	WP_004398597.1	late competence protein ComER	-
EQI27_RS13005 (EQI27_13005)	yqeM	2514493..2515236 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
EQI27_RS13010 (EQI27_13010)	rsfS	2515233..2515589 (-)	357	WP_003229971.1	ribosome silencing factor	-
EQI27_RS13015 (EQI27_13015)	yqeK	2515607..2516166 (-)	560	Protein_2522	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQI27_RS13020 (EQI27_13020)	nadD	2516156..2516725 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQI27_RS13025 (EQI27_13025)	yhbY	2516737..2517027 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQI27_RS13030 (EQI27_13030)	aroE	2517021..2517863 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=335130 EQI27_RS12995 WP_004398514.1 2512905..2513522(-) (comEA) [Bacillus subtilis strain SRCM103923]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=335130 EQI27_RS12995 WP_004398514.1 2512905..2513522(-) (comEA) [Bacillus subtilis strain SRCM103923]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39