Detailed information NTG0335050

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQH95_RS13770
• Genome accession: NZ_CP035162
• Coordinates: 2557714..2558331 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103886
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2552714..2563331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH95_RS13740 (EQH95_13740)	rpsT	2553016..2553282 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQH95_RS13745 (EQH95_13745)	holA	2553297..2554340 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
EQH95_RS13750 (EQH95_13750)	-	2554380..2554529 (-)	150	WP_003229985.1	hypothetical protein	-
EQH95_RS13755 (EQH95_13755)	yqzM	2554570..2554704 (+)	135	WP_003229983.1	YqzM family protein	-
EQH95_RS13760 (EQH95_13760)	comEC	2554744..2557074 (-)	2331	WP_069837640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQH95_RS13765 (EQH95_13765)	comEB	2557078..2557647 (-)	570	WP_003229978.1	ComE operon protein 2	-
EQH95_RS13770 (EQH95_13770)	comEA	2557714..2558331 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
EQH95_RS13775 (EQH95_13775)	comER	2558415..2559236 (+)	822	WP_032726195.1	late competence protein ComER	-
EQH95_RS13780 (EQH95_13780)	yqeM	2559302..2560045 (-)	744	WP_069837641.1	class I SAM-dependent methyltransferase	-
EQH95_RS13785 (EQH95_13785)	rsfS	2560042..2560398 (-)	357	WP_014480315.1	ribosome silencing factor	-
EQH95_RS13790 (EQH95_13790)	yqeK	2560416..2560976 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQH95_RS13795 (EQH95_13795)	nadD	2560966..2561535 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQH95_RS13800 (EQH95_13800)	yhbY	2561547..2561837 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQH95_RS13805 (EQH95_13805)	aroE	2561831..2562673 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=335050 EQH95_RS13770 WP_046160608.1 2557714..2558331(-) (comEA) [Bacillus subtilis strain SRCM103886]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=335050 EQH95_RS13770 WP_046160608.1 2557714..2558331(-) (comEA) [Bacillus subtilis strain SRCM103886]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376