Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYA12_RS05560 Genome accession   NZ_CP043197
Coordinates   1103492..1103932 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli O16:H48 strain PG20180061     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1098492..1108932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA12_RS05540 (FYA12_05540) aroP 1099490..1100863 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FYA12_RS05545 (FYA12_05545) ampE 1100906..1101760 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FYA12_RS05550 (FYA12_05550) ampD 1101757..1102308 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYA12_RS05555 (FYA12_05555) nadC 1102396..1103289 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYA12_RS05560 (FYA12_05560) pilA 1103492..1103932 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FYA12_RS05565 (FYA12_05565) pilB 1103942..1105327 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  FYA12_RS05570 (FYA12_05570) hofC 1105317..1106519 (+) 1203 WP_000157266.1 protein transport protein HofC -
  FYA12_RS05575 (FYA12_05575) guaC 1106554..1107597 (-) 1044 WP_001217338.1 GMP reductase -
  FYA12_RS22825 - 1107753..1107797 (-) 45 WP_120795372.1 protein YacM -
  FYA12_RS05580 (FYA12_05580) coaE 1107822..1108442 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=335048 FYA12_RS05560 WP_000360904.1 1103492..1103932(+) (pilA) [Escherichia coli O16:H48 strain PG20180061]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=335048 FYA12_RS05560 WP_000360904.1 1103492..1103932(+) (pilA) [Escherichia coli O16:H48 strain PG20180061]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment