Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   EPZ47_RS02035 Genome accession   NZ_CP035088
Coordinates   454684..455748 (+) Length   354 a.a.
NCBI ID   WP_135843310.1    Uniprot ID   -
Organism   Pseudomonas viciae strain 11K1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449684..460748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPZ47_RS02025 (EPZ47_02025) - 450578..451846 (+) 1269 WP_135843308.1 malic enzyme-like NAD(P)-binding protein -
  EPZ47_RS02030 (EPZ47_02030) - 452031..454478 (-) 2448 WP_135843309.1 penicillin-binding protein 1A -
  EPZ47_RS02035 (EPZ47_02035) comM 454684..455748 (+) 1065 WP_135843310.1 pilus assembly protein PilM Machinery gene
  EPZ47_RS02040 (EPZ47_02040) - 455748..456314 (+) 567 WP_135843311.1 PilN domain-containing protein -
  EPZ47_RS02045 (EPZ47_02045) pilO 456311..456934 (+) 624 WP_135843312.1 type 4a pilus biogenesis protein PilO -
  EPZ47_RS02050 (EPZ47_02050) - 456931..457458 (+) 528 WP_135843313.1 pilus assembly protein PilP -
  EPZ47_RS02055 (EPZ47_02055) pilQ 457472..459538 (+) 2067 WP_135843314.1 type IV pilus secretin PilQ Machinery gene
  EPZ47_RS02060 (EPZ47_02060) aroK 459543..460061 (+) 519 WP_123345370.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38092.52 Da        Isoelectric Point: 4.9070

>NTDB_id=334323 EPZ47_RS02035 WP_135843310.1 454684..455748(+) (comM) [Pseudomonas viciae strain 11K1]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTTSRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVLGPSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTA
EQAGQAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSTVDHILLAGGSASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=334323 EPZ47_RS02035 WP_135843310.1 454684..455748(+) (comM) [Pseudomonas viciae strain 11K1]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGGATAGACATCAGCTCCACGTCGGTGAAGCTGCTTGAGTT
GAGCCGCCAGGGTGACCGATACCGCGTCGAGTCTTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAAGGGGTGGGCCAGGCATTGTCGCGTGTGCTGGCCAAGGCCAAAACGACCTCGCGCAGCGTGGCGGTG
GCCGTGGCGGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGCCTCTCCGACGACGACATGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCATTGGATGAGGTGGCCATCGATTTCGAGGTCCTCGGCCCGTCACCGC
GCAGCGCCGAGCGGGTCGAGGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTTCGCGAGGCTGCGCTGGCGCTG
GCCGGGCTGACGGCCCGGGTGGTCGATGTTGAAGCCTACGCGCTGGAGCGCGCCTTTGGTCTGCTCGCCACGCAACTGGC
CGCGTCCCAGGAGCGGCTGACCGTGGCGGTCATCGACATCGGTGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GGATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAACTTACCGAGGAAATCCAGCGCCGCTATGGCCTGACGGCC
GAGCAGGCTGGTCAGGCAAAGAAGCAGGGTGGACTGCCGGACGATTACCTCAGCGAGGTGCTGCAGCCCTTTCGCGAGGC
CCTGGTGCAGCAGGTCTCGCGGTCCTTGCAGTTTTTCTTCGCTTCGGGCCAGTACAGCACGGTGGACCACATTTTATTGG
CCGGCGGCTCAGCGTCGGTGGCCGGCCTTGATCGGCTGATCGAACAGCGCCTGGGCACACCGACCCAGGTGGCCAACCCG
TTTACCAATATGGCCCTGAGCAGCAAGGTCAATGCCGGTGCCCTGGCCAGTGATGCTCCGGCGCTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48

Multiple sequence alignment