Detailed information NTG0334269

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EP552_RS14545
• Genome accession: NZ_CP035051
• Coordinates: 2983527..2984645 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain ABUH763
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2978527..2989645

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EP552_RS14535 (EP552_14530)	-	2981643..2982335 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
EP552_RS14540 (EP552_14535)	pilT	2982462..2983499 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EP552_RS14545 (EP552_14540)	pilU	2983527..2984645 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EP552_RS14550 (EP552_14545)	fur	2984715..2985152 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EP552_RS14555 (EP552_14550)	-	2985264..2985662 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EP552_RS14560 (EP552_14555)	-	2985717..2986040 (-)	324	WP_000442587.1	RnfH family protein	-
EP552_RS14565 (EP552_14560)	-	2986040..2987107 (-)	1068	WP_002019572.1	hypothetical protein	-
EP552_RS14575 (EP552_14570)	-	2987260..2987715 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EP552_RS14580 (EP552_14575)	-	2987968..2988816 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=334269 EP552_RS14545 WP_000347039.1 2983527..2984645(+) (pilU) [Acinetobacter baumannii strain ABUH763]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=334269 EP552_RS14545 WP_000347039.1 2983527..2984645(+) (pilU) [Acinetobacter baumannii strain ABUH763]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395